Correlation of Vascular Endothelial Growth Factor and CD105-Microvascular Density in Primary Pterygium

• Published in: Journal of Huazhong University of Science and Technology. Medical sciences 2011-08, Vol.31 (4), p.560-564
• Main Author: 张洁 张明昌 李晓青 郑恬 穆歌 刘伟 谢华桃 刘欣
• Format: Article
• Language: English
• Subjects: Adult; Aged; Antigens, CD - metabolism; CD105; Endoglin; Endothelium, Vascular - metabolism; Female; Humans; Male; Medicine; Medicine & Public Health; Microvessels - metabolism; Microvessels - pathology; Middle Aged; Pterygium - metabolism; Pterygium - pathology; Receptors, Cell Surface - metabolism; Retrospective Studies; Vascular Endothelial Growth Factor A - metabolism; VEGF; 免疫组化染色; 小学; 微血管密度; 肉; 血管内皮生长因子; 血管内皮细胞
• ISSN: 1672-0733; 1993-1352
• DOI: 10.1007/s11596-011-0490-4

The relationship between the expression of vascular endothelial growth factor（VEGF） and microvascular density（MVD） marked by CD105（CD105-MVD）,and that between CD105-MVD and the clinicopathological characteristics of primary pterygium were investigated.The streptavidin-biotin complex（SABC） immunohistochemical staining in paraffin-embedded tissues was used to detect the expression of VEGF in 23 cases of primary pterygia and 7 normal conjunctival specimens.The antibody against CD105 was used to display vascular endothelial cells,and MVD was examined by counting the CD105-positive vascular endothelial cells.The correlations of VEGF and CD105-MVD,and those of CD105-MVD and clinicopathological data were analyzed by using SPSS 12.0.The expression of VEGF was significantly increased in epithelia（P=0.000）,endothelia and stroma cells（P=0.005） in primary pterygia as compared with normal conjunctivae.The CD105-MVD in pterygia（mean 19.22±6.68） was higher than that in normal conjunctivae（mean 4.00±2.15,P=0.000）.MVD in pterygia was significantly associated with the Tan classification（P=0.000） and the VEGF expression level in the stroma（P=0.020）,but not with sex（P=0.61）,age（P=0.150） or the VEGF expression level in the epithelia（P=0.518）.Our results suggest that over-expression of VEGF and high CD105-MVD in primary pterygium may contribute to the progression by increasing angiogenesis and growth of primary pterygium.