Trichostatin A inhibits expression of cathepsins in experimental osteoarthritis

The aim of this study was to investigate the effects of trichostatin A (TSA) on expression of cathepsins in cartilage in experimental osteoarthritis (OA). OA was induced in 18 rabbits by bilateral anterior cruciate ligament transection (ACLT). Four weeks after surgery, rabbits received intra-articul...

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Bibliographic Details
Published in:Rheumatology international 2011-10, Vol.31 (10), p.1325-1331
Main Authors: Chen, Wei-Ping, Bao, Jia-Peng, Tang, Jin-Li, Hu, Peng-Fei, Wu, Li-Dong
Format: Article
Language:English
Subjects:
Animals
Cathepsins - antagonists & inhibitors
Cathepsins - genetics
Disease Models, Animal
Gene Expression Regulation, Enzymologic - physiology
Hydroxamic Acids - pharmacology
Male
Medicine
Medicine & Public Health
Original Article
Osteoarthritis, Knee - drug therapy
Osteoarthritis, Knee - enzymology
Protease Inhibitors - pharmacology
Rabbits
Rheumatology
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Summary:The aim of this study was to investigate the effects of trichostatin A (TSA) on expression of cathepsins in cartilage in experimental osteoarthritis (OA). OA was induced in 18 rabbits by bilateral anterior cruciate ligament transection (ACLT). Four weeks after surgery, rabbits received intra-articular injection with TSA dissolved in the dimethylsulphoxide (DMSO) in the right knees and DMSO in the left knees once a week for 5 weeks. Rabbits were killed 7 days after the last injection. The knee joints were assessed by morphological and histological examination. Messenger RNA expression of cathepsins K, B, L, S and cystatin C was studied by real-time PCR. TSA inhibited the expression of cathepsins K, B, L, S and cystatin C accompanied with the less degradation in cartilage. The results suggest that TSA exhibits protective effects against cartilage degradation in rabbits with OA and the effects may be associated with the inhibition of cathepsins.
ISSN:0172-8172
1437-160X
DOI:10.1007/s00296-010-1481-7