Electrophoretic isolation of saponin fractions from Saponinum album and their evaluation in synergistically enhancing the receptor-specific cytotoxicity of targeted toxins

Saponinum album (SA) is a commercial mixture of saponins isolated from Gypsophila species. In the previously published work, we reported that SA dramatically improves the inhibition of tumor growth by targeted toxins in mice in a synergistic way. Here we report a simplified electrophoretic method fo...

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Bibliographic Details
Published in:Electrophoresis 2011-11, Vol.32 (21), p.3085-3089
Main Authors: Thakur, Mayank, Weng, Alexander, Bachran, Diana, Riese, Sebastian B., Böttger, Stefan, Melzig, Matthias F., Fuchs, Hendrik
Format: Article
Language:English
Subjects:
Animals
Caryophyllaceae - chemistry
Cell Survival - drug effects
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Drug Synergism
Electrophoresis - methods
Electrophoretic mobility
Epidermal growth factor
Epidermal Growth Factor - metabolism
Gel electrophoresis
Humans
Immunotoxins - metabolism
Mice
NIH 3T3 Cells
Saponin isolation
Saponins - chemistry
Saponins - isolation & purification
Saponins - pharmacology
Spectrometry, Mass, Electrospray Ionization
Substrate Specificity
Targeted toxin
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Summary:Saponinum album (SA) is a commercial mixture of saponins isolated from Gypsophila species. In the previously published work, we reported that SA dramatically improves the inhibition of tumor growth by targeted toxins in mice in a synergistic way. Here we report a simplified electrophoretic method for the isolation of a highly effective fraction of SA with a relative electrophoretic mobility to the dye front (Rf) of 0.63 from the mixture. In total, four different fractions were separated at a preparative scale, and evaluated by ESI‐MS, HPLC and TLC analysis. Electrophoretic mobility and electrochemical properties of the different fractions of saponins from SA were set into relation to their ability to enhance the cytotoxicity of epidermal growth factor (EGF)‐based targeted toxins. We here treated HER‐14 cells, which are NIH‐3T3 Swiss mouse embryo cells transfected with the human EGF receptor. Untransfected NIH‐3T3 cells served as control. The major bulk of SA (72.3%) (Rf=0.78) migrated the farthest and was found to be significantly ineffective (p
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.201100155