Characterization of hepatitis C virus in chronic hepatitis patients: genotypes in the State of Amazonas, Brazil

In the State of Amazonas, data regarding the prevalence of different genotypes of hepatitis C virus remains scarce. The genotype of 69 HCV positive patients was determined. An in-house standardized nested-PCR was used to detect HCV RNA. Genotype assignment was based on type-specific motifs on the se...

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Bibliographic Details
Published in:Revista da Sociedade Brasileira de Medicina Tropical 2011-10, Vol.44 (5), p.638-640
Main Authors: Araújo, Ana Ruth, Almeida, Carlos Mauríco de, Fraporti, Liziara, Garcia, Nadja, Lima, Tatiane Amábili de, Maia, Laura Patrícia Viana, Torres, Kátia Luz, Tarragô, Andréa Monteiro, Victória, Flamir, Victória, Marilu, Tateno, Adriana, Levi, José Eduardo, Talhari, Sinésio, Malheiro, Adriana
Format: Article
Language:Portuguese
Subjects:
Brazil
Female
Genotype
Genotypes
Hepacivirus - genetics
Hepatitis
Hepatitis C, Chronic - virology
Humans
Male
Middle Aged
Reverse Transcriptase Polymerase Chain Reaction
RNA, Viral - analysis
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Summary:In the State of Amazonas, data regarding the prevalence of different genotypes of hepatitis C virus remains scarce. The genotype of 69 HCV positive patients was determined. An in-house standardized nested-PCR was used to detect HCV RNA. Genotype assignment was based on type-specific motifs on the sequenced amplicons delimited by primers HC11/HC18 from the 5' untranslated region. Of the 69 patients studied, 65.2% were male and 34.8% were female. Genotype 1 showed the greatest prevalence, followed by 3 and 2. These data suggesting that Manaus is the point of arrival of HCV in the State of Amazonas.
ISSN:0037-8682
1678-9849
DOI:10.1590/S0037-86822011000500024