Activation of hypoxia-inducible factor-1 ameliorates postischemic renal injury via inducible nitric oxide synthase

• Published in: Molecular and cellular biochemistry 2011-12, Vol.358 (1-2), p.287-295
• Main Authors: Zhang, Xiao-Li, Yan, Zhen-Wen, Sheng, Wei-Wen, Xiao, Jing, Zhang, Zhen-Xing, Ye, Zhi-Bin
• Format: Article
• Language: English
• Subjects: Amino Acids, Dicarboxylic - pharmacology; Analysis; Animals; Apoptosis - drug effects; Biochemistry; Biomedical and Life Sciences; Cardiology; Creatinine - blood; Enzymes; Erythropoietin; Hydroxylases; Hypoxia; Hypoxia-Inducible Factor 1 - metabolism; Intermediate filament proteins; Ischemia; Kidney - drug effects; Kidney - enzymology; Kidney - pathology; Kidneys; Life Sciences; Male; Medical Biochemistry; Mice; Mice, Inbred C57BL; Nitric oxide; Nitric Oxide Synthase Type II - antagonists & inhibitors; Nitric Oxide Synthase Type II - metabolism; Oncology; Pharmacology; Protective Agents - pharmacology; Protein Stability - drug effects; Proteins; Renal function; Reperfusion Injury - blood; Reperfusion Injury - enzymology; Reperfusion Injury - pathology; Sulfides - pharmacology; Vimentin - metabolism
• ISSN: 0300-8177; 1573-4919
• DOI: 10.1007/s11010-011-0979-y

Hypoxia-inducible factor-1 (HIF-1) could ameliorate renal ischemia reperfusion injury (IRI), but the underlying mechanism remains elusive. In the current study, we aim to investigate the possible role of prolyl hydroxylases inhibitor dimethyloxalylglycine (DMOG) in inducing delayed preconditioning-like effects against IRI. Mice were divided into four groups ( n  = 6): sham group; IRI group; DMOG group: pretreated with DMOG 24 h before IRI; and GW274150 + DMOG group: pretreated with DMOG followed by iNOS inhibitor GW274150 treatment 24 h before IRI. The results showed that the protein level of HIF-1a and the expression of its targets inducible nitric oxide synthase (iNOS), erythropoietin, and heme oxygenase-1 were obviously increased after administration of DMOG. Histological analysis of renal function showed improvement in tubulointerstitial injury due to ischemia by delayed preconditioning with DMOG. GW274150 antagonized the delayed renal protection afforded by DMOG as reflected by deteriorated renal dysfunction, aggravated histological injury, increased renal cell apoptosis, and increased vimentin expression in the kidney. In conclusion, our data demonstrate that DMOG pretreatment induces delayed renal protection against IRI in mice and the beneficial effects are mitigated by pharmacological inhibition of iNOS, suggesting that the protective effects derived from HIF-1 activation via DMOG in the kidney are partially mediated by iNOS.