Nitric oxide content is associated with tolerance to bicarbonate-induced chlorosis in micropropagated Prunus explants

• Published in: Journal of plant physiology 2011-09, Vol.168 (13), p.1543-1549
• Main Authors: Cellini, Antonio, Corpas, Francisco Javier, Barroso, Juan Bautista, Masia, Andrea
• Format: Article
• Language: English
• Subjects: Adaptation, Physiological - physiology; antioxidants; Antioxidants - metabolism; Bicarbonate; Bicarbonates; Bicarbonates - pharmacology; Biological and medical sciences; calcareous soils; Chimera; Chlorophyll - metabolism; Chlorosis; cultivars; culture media; explants; Fruit - drug effects; Fruit - genetics; Fruit - metabolism; fruit trees; Fundamental and applied biological sciences. Psychology; Genotype; hybrids; Hydrogen Peroxide - metabolism; Hydrogen-Ion Concentration; Iron; Iron - deficiency; Iron - metabolism; Iron and steel plants; metabolism; micropropagation; Nitric oxide; Nitric Oxide - metabolism; nitrogen; nutrient deficiencies; Oxidative Stress; Peach rootstocks; Peaches; Phenotype; Plant Leaves - drug effects; Plant Leaves - genetics; Plant Leaves - metabolism; Plant physiology and development; Plant Roots - drug effects; Plant Roots - genetics; Plant Roots - metabolism; Prunus; Prunus - drug effects; Prunus - genetics; Prunus - physiology; Reactive nitrogen species; Reactive Nitrogen Species - metabolism; Reactive Oxygen Species - metabolism; rootstocks; Soil; Soils; Stress, Physiological; Stresses; Tissue cultures, protoplasts; Tolerances
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/j.jplph.2011.02.008

Iron (Fe) chlorosis is a common nutritional deficiency in fruit trees grown in calcareous soils. Grafting on tolerant rootstocks is the most efficient practice to cope with it. In the present work, three Prunus hybrid genotypes, commonly used as peach rootstocks, and one peach cultivar were cultivated with bicarbonate in the growth medium. Parameters describing oxidative stress and the metabolism of reactive nitrogen species were studied. Lower contents of nitric oxide and a decreased nitrosoglutathione reductase activity were found in the most sensitive genotypes, characterized by higher oxidative stress and reduced antioxidant defense. In the peach cultivar, which behaved as a tolerant genotype, a specifically nitrated polypeptide was found.