Design, simulation and characterisation of integrated optics for a microfabricated flow cytometer

Flow cytometry is widely used for analyzing micro-particles such as cells and bacteria. Microfabricated flow cytometers promise reduced instrument size and cost with increased robustness and have application in medicine, life sciences and environmental metrology. Further miniaturisation and robustne...

Full description

Saved in:
Bibliographic Details
Published in:Optics communications 2010-05, Vol.283 (9), p.1987-1992
Main Authors: Barat, David, Benazzi, Giuseppe, Mowlem, Matthew Charles, Ruano, Jesus Miguel, Morgan, Hywel
Format: Article
Language:English
Subjects:
Bacteria
Collimation of light
Design engineering
Diffraction and scattering
Exact sciences and technology
Flow cytometry
Fluorescence
Fundamental areas of phenomenology (including applications)
Integrated optics
Optical elements, devices, and systems
Optical losses
Optical waveguides and coupleurs
Optics
Physics
Robustness
Sheaths
Simulation
Wave optics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Flow cytometry is widely used for analyzing micro-particles such as cells and bacteria. Microfabricated flow cytometers promise reduced instrument size and cost with increased robustness and have application in medicine, life sciences and environmental metrology. Further miniaturisation and robustness can be achieved if integrated optics are used instead of traditional free space optics. We present designs simulation and experimental characterisation of integrated optics for a microfabricated cytometer made from SU-8 resin on a glass substrate. The optics constructed from combinations of optical fibres (positioned with microgrooves), waveguides, and microlenses enable analysis of scattered light and fluorescence from particles positioned near the centre of a microchannel using one dimensional sheath flow. Four different methods for directing the incident light onto the particles are examined and the optimum design discussed.
ISSN:0030-4018
1873-0310
DOI:10.1016/j.optcom.2009.12.046