Improved characterization of tomato polyphenols using liquid chromatography/electrospray ionization linear ion trap quadrupole Orbitrap mass spectrometry and liquid chromatography/electrospray ionization tandem mass spectrometry

Tomato (Lycopersicon esculentum Mill.) is the second most important fruit crop worldwide. Tomatoes are a key component in the Mediterranean diet, which is strongly associated with a reduced risk of chronic degenerative diseases. In this work, we use a combination of mass spectrometry (MS) techniques...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2010-10, Vol.24 (20), p.2986-2992
Main Authors: Vallverdú-Queralt, Anna, Jáuregui, Olga, Medina-Remón, Alexander, Andrés-Lacueva, Cristina, Lamuela-Raventós, Rosa M.
Format: Article
Language:English
Subjects:
Benzoic Acid - chemistry
Chromatography, High Pressure Liquid
Coumaric Acids - chemistry
Diets
Flavonoids - chemistry
Ionization
Liquid chromatography
Lycopersicon esculentum - chemistry
Mass spectrometry
Polyphenols
Precursors
Quadrupoles
Spectrometry
Spectrometry, Mass, Electrospray Ionization - methods
Tandem Mass Spectrometry - methods
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Summary:Tomato (Lycopersicon esculentum Mill.) is the second most important fruit crop worldwide. Tomatoes are a key component in the Mediterranean diet, which is strongly associated with a reduced risk of chronic degenerative diseases. In this work, we use a combination of mass spectrometry (MS) techniques with negative ion detection, liquid chromatography/electrospray ionization linear ion trap quadrupole‐Orbitrap‐mass spectrometry (LC/ESI‐LTQ‐Orbitrap‐MS) and liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS) on a triple quadrupole, for the identification of the constituents of tomato samples. First, we tested for the presence of polyphenolic compounds through generic MS/MS experiments such as neutral loss and precursor ion scans on the triple quadrupole system. Confirmation of the compounds previously identified was accomplished by injection into the high‐resolution system (LTQ‐Orbitrap) using accurate mass measurements in MS, MS2 and MS3 modes. In this way, 38 compounds were identified in tomato samples with very good mass accuracy (
ISSN:0951-4198
1097-0231
1097-0231
DOI:10.1002/rcm.4731