Retention prediction in reversed-phase liquid chromatography systems with methanol/water mobile phases containing different alkanols as additives

In an effort to gain enhancement of selectivity in reversed-phase liquid chromatography, retention was tuned in this study by introducing short and medium straight-chained-length alkanol additives (methanol (MeOH), ethanol, 1-propanol, 1-butanol, 1-pentanol, 1-hexanol or 1-heptanol) at low concentra...

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Bibliographic Details
Published in:Journal of separation science 2011-02, Vol.34 (3), p.255-259
Main Authors: Pappa-Louisi, Adriani, Agrafiotou, Panagiota, Fasoula, Stella
Format: Article
Language:English
Subjects:
Additives
Alkanol additive-methanol-water mobile phases
Analytical chemistry
Carbon
Chemistry
Chromatographic methods and physical methods associated with chromatography
Chromatography
Ethyl alcohol
Exact sciences and technology
Fittings
Liquid chromatography
Low concentrations
Mathematical models
Methyl alcohol
Modeling
Other chromatographic methods
Phases
Retention
Retention prediction
Reversed phase liquid chromatography
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Summary:In an effort to gain enhancement of selectivity in reversed-phase liquid chromatography, retention was tuned in this study by introducing short and medium straight-chained-length alkanol additives (methanol (MeOH), ethanol, 1-propanol, 1-butanol, 1-pentanol, 1-hexanol or 1-heptanol) at low concentrations in mobile phases containing MeOH as the main organic solvent. A six-parameter retention model considering simultaneously the contents of the main organic modifier and of the alcohol additive as well as of the number of alkyl chain of additive was developed by a direct combination of equations expressing separately a linear dependence of the retention upon each of these factors. The effectiveness of the above model was tested in the retention prediction of a mixture of six alkylbenzenes under isocratic conditions with mobile phases containing as an additive any member of the homologues series of alkanols (with 1-7 carbon atoms) at different low concentrations in a wide range of MeOH-water mixtures. The prediction was excellent in all cases even when the alkanol additives used in experiments for the fitting procedure are different than those used in chromatographic runs done for testing the prediction ability of the proposed model.
ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.201000725