A new open tubular capillary microextraction and sweeping for the analysis of super low concentration of hydrophobic compounds

A sample pre‐concentration method based on the in‐line coupling of in‐tube solid‐phase microextraction and electrophoretic sweeping was developed for the analysis of hydrophobic compounds. The sample pre‐concentration and electrophoretic separation processes were simply and sequentially carried out...

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Bibliographic Details
Published in:Journal of separation science 2010-10, Vol.33 (20), p.3221-3230
Main Authors: Xia, Zhining, Gan, Tingting, Chen, Hua, Lv, Rui, Wei, Weili, Yang, Fengqing
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Capillarity
Enrichment
Flow rate
General pharmacology
Hydrophobic and Hydrophilic Interactions
Hydrophobic compounds
Ibuprofen
Ibuprofen - blood
Ibuprofen - chemistry
In-line coupling
In-tube solid-phase microextraction
Indomethacin - blood
Indomethacin - chemistry
Loratadine - blood
Loratadine - chemistry
Medical sciences
Optimization
Pharmacology. Drug treatments
Rabbits
Sample pre-concentration
Sampling
Separation
Solid Phase Microextraction - methods
Sweeping
Temperature
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Summary:A sample pre‐concentration method based on the in‐line coupling of in‐tube solid‐phase microextraction and electrophoretic sweeping was developed for the analysis of hydrophobic compounds. The sample pre‐concentration and electrophoretic separation processes were simply and sequentially carried out with a (35%‐phenyl)‐methylpolysiloxane‐coated capillary. The developed method was validated and applied to enrich and separate several pharmaceuticals including loratadine, indomethacin, ibuprofen and doxazosin. Several parameters of microextration were investigated such as temperature, pH and eluant. And the concentration of microemulsion that influences separation efficiency and microextraction efficiency were also studied. Central composite design was applied for the optimization of sampling flow rate and sampling time that interact in a very complex way with each other. The precision, sensitivity and recovery of the method were investigated. Under the optimal conditions, the maximum enrichment factors for loratadine, indomethacin, ibuprofen and doxazosin in aqueous solutions are 1355, 571, 523 and 318, respectively. In addition, the developed method was applied to determine loratadine in rabbit blood sample.
ISSN:1615-9306
1615-9314
1615-9314
DOI:10.1002/jssc.201000282