A rapid and specific derivatization procedure to identify acyl-glucuronides by mass spectrometry

A simple procedure is described to identify acyl‐glucuronides by coupled liquid chromatography/mass spectrometry after derivatization to a hydroxamic acid with hydroxylamine. The reaction specificity obviates the need for isolation of the acyl‐glucuronide from an extract. Glucuronides derived from c...

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Bibliographic Details
Published in:Rapid communications in mass spectrometry 2010-07, Vol.24 (14), p.2109-2121
Main Authors: Vaz, Alfin D. N., Wang, Wei Wei, Bessire, Andrew J., Sharma, Raman, Hagen, Anne E.
Format: Article
Language:English
Subjects:
Amines
Carbamates - chemistry
Derivatives
Fingerprints
Glucuronides - chemistry
Hydroxamic Acids - chemistry
Hydroxylamine - chemistry
Inert
Liquid chromatography
Mass spectrometry
Mass Spectrometry - methods
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Summary:A simple procedure is described to identify acyl‐glucuronides by coupled liquid chromatography/mass spectrometry after derivatization to a hydroxamic acid with hydroxylamine. The reaction specificity obviates the need for isolation of the acyl‐glucuronide from an extract. Glucuronides derived from carbamic acids, and alkyl‐ and aromatic amines, are inert to the derivatization reaction conditions, making the hydroxamic acid derivative a fingerprint for acyl‐glucuronides. Copyright © 2010 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
1097-0231
DOI:10.1002/rcm.4621