Production of in vitro haploid plants from in situ induced haploid embryos in winter squash (Cucurbita maxima Duchesne ex Lam.) via irradiated pollen

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2010-09, Vol.102 (3), p.267-277
• Main Authors: Kurtar, Ertan Sait, Balkaya, Ahmet
• Format: Article
• Language: English
• Subjects: application timing; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cucurbita maxima; developmental stages; dosage; duration; embryo (plant); embryo culture; embryogenesis; Embryos; Fruits; Fundamental and applied biological sciences. Psychology; gamma radiation; Gamma rays; genotype; Genotypes; haploidy; harvest date; in vitro regeneration; Irradiation; Life Sciences; necrosis; Original Paper; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant reproduction; Plant Sciences; Plantlets; Pollen; Pollination; Radiation; Radiation dosage; Seeds; squashes; Winter
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-010-9729-1

The influence of pollen irradiation on the production of in vitro haploid plants from in situ induced haploid embryos was investigated in winter squash (Cucurbita maxima Duchesne ex Lam.). Pollen were irradiated at different gamma-ray doses (50, 100, 200 and 300 Gray) and durations (9, 11, 15, 21, and 28 July). Production of in vitro haploid plantlets was influenced by irradiation dose, irradiation duration, genotype, and embryo type and embryo stage. Embryos were only obtained from lower irradiation doses (50 Gray and 100 Gray) and earlier irradiation durations (9, 11, and 15 July). The greatest embryo number per fruit was procured from “G14” and “55SI06” genotypes at 50 Gray gamma-ray dose. Necrotic embryos were higher than normal embryos at delayed harvest times (5 and 6 weeks after the pollination). The convenient harvest time for embryo rescue was observed about 4 weeks (between 25 and 30 days) after pollination. All cotyledon and amorphous embryos had only diploid plants while late-torpedo, arrow-tip, and pro-cotyledon embryos produced 33.3, 50.0, and 66.7% haploid plant. The frequency of haploid plantlets was 0.11, 1.17, 10.96 and 0.28 per 100 seeds, 100 embryos, 100 plantlets and a fruit at 50 Gray gamma-ray dose, respectively.