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Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells
Background: Lactobacilli represent a major component of the human microbiota. In this study we investigated whether and how Lactobacillus fermentum inhibits the proinflammatory responses of human epithelial cells on Yersinia enterocolitica infection. Methods: Human epithelial cells were exposed to Y...
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| Published in: | Inflammatory bowel diseases 2007-01, Vol.13 (1), p.83-90 |
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| container_title | Inflammatory bowel diseases |
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| creator | Frick, Julia‐Stefanie Schenk, Katrin Quitadamo, Matteo Kahl, Frauke Köberle, Martin Bohn, Erwin Aepfelbacher, Martin Autenrieth, Ingo B. |
| description | Background: Lactobacilli represent a major component of the human microbiota. In this study we investigated whether and how Lactobacillus fermentum inhibits the proinflammatory responses of human epithelial cells on Yersinia enterocolitica infection.
Methods: Human epithelial cells were exposed to Y. enterocolitica pYV− or L. fermentum or to both strains, combinations of heat‐killed L. fermentum or supernatant of L. fermentum cultures and Y. enterocolitica. The modulation of Y. enterocolitica induced IL‐8 levels in the culture supernatants was determined and activation of Rac, p38, and NF‐κB was investigated.
Results: Exposure of human epithelial cells to L. fermentum does not induce NF‐κB activation and subsequent IL‐8 secretion in HeLa cells, whereas Y. enterocolitica induces NF‐κB activation and high levels of IL‐8. Viable L. fermentum, supernatant of L. fermentum cultures, but not heat‐killed L. fermentum, inhibited IL‐8 secretion of HeLa cells triggered by Y. enterocolitica. Lactobacillus fermentum‐exposed HeLa cells showed decreased Rac, p38, and NF‐κB activation after Y. enterocolitica infection. Treatment of L. fermentum supernatants with phospholipase C abolished the inhibitory effect, indicating that a secreted phospholipid mediates the antiinflammatory properties of L. fermentum. Adhesion to or invasion of Y. enterocolitica into epithelial cells was not altered by coincubation with L. fermentum.
Conclusion: Our results lead to the conclusion that L. fermentum inhibits the Y. enterocolitica‐induced IL‐8 production by a possibly secreted phospholipid of |
| doi_str_mv | 10.1002/ibd.20009 |
| format | article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_902334599</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>902334599</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3909-277f718904e77c01494ad335a30e94ea3674faf783e3f094b34d4187ce3da4483</originalsourceid><addsrcrecordid>eNp9kT2PFDEMhiME4j6g4A-gVKAr5s4z8U4mJXd8nbQSDRRUI2_G0QVlJkuSEdp_T45diQrkwi4eP7L8CvGqhesWoLvxu-m6AwDzRJy3G9U3OCA-rTPooQFjhjNxkfOPitYyz8VZqzvoe1TnIm3Jlrgj60NYs3ScZl7KOksqhZeVCmdZHljuU_SLCzTPVGI6SHaObZHRye-csl88ybrHKdoYfPGWZFzkwzrTInnvqyF4CtJyCPmFeOYoZH556pfi28cPX-8-N9svn-7v3m0bqwyYptPa6XYwgKy1hRYN0qTUhhSwQSbVa3Tk9KBYOTC4UzhhO2jLaiLEQV2Kt0dvvf3nyrmMs8-PF9DCcc2jgU4p3BhTyTf_JfsBoQfcVPDqCNoUc07sxn3yM6XD2ML4GMVYoxj_RFHZ1yfpupt5-kuefl-BmyPwywc-_Ns03t--Pyp_A2kslBY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>68406045</pqid></control><display><type>article</type><title>Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells</title><source>Oxford University Press:Jisc Collections:OUP Read and Publish 2024-2025 (2024 collection) (Reading list)</source><creator>Frick, Julia‐Stefanie ; Schenk, Katrin ; Quitadamo, Matteo ; Kahl, Frauke ; Köberle, Martin ; Bohn, Erwin ; Aepfelbacher, Martin ; Autenrieth, Ingo B.</creator><creatorcontrib>Frick, Julia‐Stefanie ; Schenk, Katrin ; Quitadamo, Matteo ; Kahl, Frauke ; Köberle, Martin ; Bohn, Erwin ; Aepfelbacher, Martin ; Autenrieth, Ingo B.</creatorcontrib><description>Background: Lactobacilli represent a major component of the human microbiota. In this study we investigated whether and how Lactobacillus fermentum inhibits the proinflammatory responses of human epithelial cells on Yersinia enterocolitica infection.
Methods: Human epithelial cells were exposed to Y. enterocolitica pYV− or L. fermentum or to both strains, combinations of heat‐killed L. fermentum or supernatant of L. fermentum cultures and Y. enterocolitica. The modulation of Y. enterocolitica induced IL‐8 levels in the culture supernatants was determined and activation of Rac, p38, and NF‐κB was investigated.
Results: Exposure of human epithelial cells to L. fermentum does not induce NF‐κB activation and subsequent IL‐8 secretion in HeLa cells, whereas Y. enterocolitica induces NF‐κB activation and high levels of IL‐8. Viable L. fermentum, supernatant of L. fermentum cultures, but not heat‐killed L. fermentum, inhibited IL‐8 secretion of HeLa cells triggered by Y. enterocolitica. Lactobacillus fermentum‐exposed HeLa cells showed decreased Rac, p38, and NF‐κB activation after Y. enterocolitica infection. Treatment of L. fermentum supernatants with phospholipase C abolished the inhibitory effect, indicating that a secreted phospholipid mediates the antiinflammatory properties of L. fermentum. Adhesion to or invasion of Y. enterocolitica into epithelial cells was not altered by coincubation with L. fermentum.
Conclusion: Our results lead to the conclusion that L. fermentum inhibits the Y. enterocolitica‐induced IL‐8 production by a possibly secreted phospholipid of <10 kDa molecular weight. These data suggest that L. fermentum may have probiotic properties modulating intestinal inflammatory responses and might offer new therapeutic strategies in the treatment of intestinal inflammatory diseases.
(Inflamm Bowel Dis 2007;13:83–90)</description><identifier>ISSN: 1078-0998</identifier><identifier>EISSN: 1536-4844</identifier><identifier>DOI: 10.1002/ibd.20009</identifier><identifier>PMID: 17206643</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Bacterial Adhesion ; Cell culture ; Cells, Cultured ; Culture Media, Conditioned ; Data processing ; Endopeptidase K - pharmacology ; Epithelial cells ; Epithelial Cells - metabolism ; Epithelial Cells - microbiology ; Glycerophospholipids - chemistry ; Glycerophospholipids - pharmacology ; HeLa Cells ; Humans ; IL‐8 ; Infection ; Inflammation ; Inflammation Mediators - metabolism ; Inflammatory bowel diseases ; Inflammatory diseases ; Interleukin 8 ; Interleukin-8 - secretion ; Intestine ; Iron Compounds - pharmacology ; Lactobacilli ; Lactobacillus ; Lactobacillus fermentum ; Lactobacillus fermentum - physiology ; Molecular weight ; NF- Kappa B protein ; NF-kappa B - metabolism ; p38 Mitogen-Activated Protein Kinases - metabolism ; Phospholipase C ; Phospholipids ; probiotics ; rac1 GTP-Binding Protein - metabolism ; Yersinia ; Yersinia enterocolitica ; Yersinia enterocolitica - pathogenicity ; Yersinia Infections - metabolism</subject><ispartof>Inflammatory bowel diseases, 2007-01, Vol.13 (1), p.83-90</ispartof><rights>Copyright © 2006 Crohn's & Colitis Foundation of America, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3909-277f718904e77c01494ad335a30e94ea3674faf783e3f094b34d4187ce3da4483</citedby><cites>FETCH-LOGICAL-c3909-277f718904e77c01494ad335a30e94ea3674faf783e3f094b34d4187ce3da4483</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17206643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Frick, Julia‐Stefanie</creatorcontrib><creatorcontrib>Schenk, Katrin</creatorcontrib><creatorcontrib>Quitadamo, Matteo</creatorcontrib><creatorcontrib>Kahl, Frauke</creatorcontrib><creatorcontrib>Köberle, Martin</creatorcontrib><creatorcontrib>Bohn, Erwin</creatorcontrib><creatorcontrib>Aepfelbacher, Martin</creatorcontrib><creatorcontrib>Autenrieth, Ingo B.</creatorcontrib><title>Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells</title><title>Inflammatory bowel diseases</title><addtitle>Inflamm Bowel Dis</addtitle><description>Background: Lactobacilli represent a major component of the human microbiota. In this study we investigated whether and how Lactobacillus fermentum inhibits the proinflammatory responses of human epithelial cells on Yersinia enterocolitica infection.
Methods: Human epithelial cells were exposed to Y. enterocolitica pYV− or L. fermentum or to both strains, combinations of heat‐killed L. fermentum or supernatant of L. fermentum cultures and Y. enterocolitica. The modulation of Y. enterocolitica induced IL‐8 levels in the culture supernatants was determined and activation of Rac, p38, and NF‐κB was investigated.
Results: Exposure of human epithelial cells to L. fermentum does not induce NF‐κB activation and subsequent IL‐8 secretion in HeLa cells, whereas Y. enterocolitica induces NF‐κB activation and high levels of IL‐8. Viable L. fermentum, supernatant of L. fermentum cultures, but not heat‐killed L. fermentum, inhibited IL‐8 secretion of HeLa cells triggered by Y. enterocolitica. Lactobacillus fermentum‐exposed HeLa cells showed decreased Rac, p38, and NF‐κB activation after Y. enterocolitica infection. Treatment of L. fermentum supernatants with phospholipase C abolished the inhibitory effect, indicating that a secreted phospholipid mediates the antiinflammatory properties of L. fermentum. Adhesion to or invasion of Y. enterocolitica into epithelial cells was not altered by coincubation with L. fermentum.
Conclusion: Our results lead to the conclusion that L. fermentum inhibits the Y. enterocolitica‐induced IL‐8 production by a possibly secreted phospholipid of <10 kDa molecular weight. These data suggest that L. fermentum may have probiotic properties modulating intestinal inflammatory responses and might offer new therapeutic strategies in the treatment of intestinal inflammatory diseases.
(Inflamm Bowel Dis 2007;13:83–90)</description><subject>Bacterial Adhesion</subject><subject>Cell culture</subject><subject>Cells, Cultured</subject><subject>Culture Media, Conditioned</subject><subject>Data processing</subject><subject>Endopeptidase K - pharmacology</subject><subject>Epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - microbiology</subject><subject>Glycerophospholipids - chemistry</subject><subject>Glycerophospholipids - pharmacology</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>IL‐8</subject><subject>Infection</subject><subject>Inflammation</subject><subject>Inflammation Mediators - metabolism</subject><subject>Inflammatory bowel diseases</subject><subject>Inflammatory diseases</subject><subject>Interleukin 8</subject><subject>Interleukin-8 - secretion</subject><subject>Intestine</subject><subject>Iron Compounds - pharmacology</subject><subject>Lactobacilli</subject><subject>Lactobacillus</subject><subject>Lactobacillus fermentum</subject><subject>Lactobacillus fermentum - physiology</subject><subject>Molecular weight</subject><subject>NF- Kappa B protein</subject><subject>NF-kappa B - metabolism</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Phospholipase C</subject><subject>Phospholipids</subject><subject>probiotics</subject><subject>rac1 GTP-Binding Protein - metabolism</subject><subject>Yersinia</subject><subject>Yersinia enterocolitica</subject><subject>Yersinia enterocolitica - pathogenicity</subject><subject>Yersinia Infections - metabolism</subject><issn>1078-0998</issn><issn>1536-4844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><recordid>eNp9kT2PFDEMhiME4j6g4A-gVKAr5s4z8U4mJXd8nbQSDRRUI2_G0QVlJkuSEdp_T45diQrkwi4eP7L8CvGqhesWoLvxu-m6AwDzRJy3G9U3OCA-rTPooQFjhjNxkfOPitYyz8VZqzvoe1TnIm3Jlrgj60NYs3ScZl7KOksqhZeVCmdZHljuU_SLCzTPVGI6SHaObZHRye-csl88ybrHKdoYfPGWZFzkwzrTInnvqyF4CtJyCPmFeOYoZH556pfi28cPX-8-N9svn-7v3m0bqwyYptPa6XYwgKy1hRYN0qTUhhSwQSbVa3Tk9KBYOTC4UzhhO2jLaiLEQV2Kt0dvvf3nyrmMs8-PF9DCcc2jgU4p3BhTyTf_JfsBoQfcVPDqCNoUc07sxn3yM6XD2ML4GMVYoxj_RFHZ1yfpupt5-kuefl-BmyPwywc-_Ns03t--Pyp_A2kslBY</recordid><startdate>200701</startdate><enddate>200701</enddate><creator>Frick, Julia‐Stefanie</creator><creator>Schenk, Katrin</creator><creator>Quitadamo, Matteo</creator><creator>Kahl, Frauke</creator><creator>Köberle, Martin</creator><creator>Bohn, Erwin</creator><creator>Aepfelbacher, Martin</creator><creator>Autenrieth, Ingo B.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7QL</scope><scope>7T5</scope><scope>C1K</scope><scope>H94</scope></search><sort><creationdate>200701</creationdate><title>Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells</title><author>Frick, Julia‐Stefanie ; Schenk, Katrin ; Quitadamo, Matteo ; Kahl, Frauke ; Köberle, Martin ; Bohn, Erwin ; Aepfelbacher, Martin ; Autenrieth, Ingo B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3909-277f718904e77c01494ad335a30e94ea3674faf783e3f094b34d4187ce3da4483</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Bacterial Adhesion</topic><topic>Cell culture</topic><topic>Cells, Cultured</topic><topic>Culture Media, Conditioned</topic><topic>Data processing</topic><topic>Endopeptidase K - pharmacology</topic><topic>Epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - microbiology</topic><topic>Glycerophospholipids - chemistry</topic><topic>Glycerophospholipids - pharmacology</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>IL‐8</topic><topic>Infection</topic><topic>Inflammation</topic><topic>Inflammation Mediators - metabolism</topic><topic>Inflammatory bowel diseases</topic><topic>Inflammatory diseases</topic><topic>Interleukin 8</topic><topic>Interleukin-8 - secretion</topic><topic>Intestine</topic><topic>Iron Compounds - pharmacology</topic><topic>Lactobacilli</topic><topic>Lactobacillus</topic><topic>Lactobacillus fermentum</topic><topic>Lactobacillus fermentum - physiology</topic><topic>Molecular weight</topic><topic>NF- Kappa B protein</topic><topic>NF-kappa B - metabolism</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Phospholipase C</topic><topic>Phospholipids</topic><topic>probiotics</topic><topic>rac1 GTP-Binding Protein - metabolism</topic><topic>Yersinia</topic><topic>Yersinia enterocolitica</topic><topic>Yersinia enterocolitica - pathogenicity</topic><topic>Yersinia Infections - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Frick, Julia‐Stefanie</creatorcontrib><creatorcontrib>Schenk, Katrin</creatorcontrib><creatorcontrib>Quitadamo, Matteo</creatorcontrib><creatorcontrib>Kahl, Frauke</creatorcontrib><creatorcontrib>Köberle, Martin</creatorcontrib><creatorcontrib>Bohn, Erwin</creatorcontrib><creatorcontrib>Aepfelbacher, Martin</creatorcontrib><creatorcontrib>Autenrieth, Ingo B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Inflammatory bowel diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Frick, Julia‐Stefanie</au><au>Schenk, Katrin</au><au>Quitadamo, Matteo</au><au>Kahl, Frauke</au><au>Köberle, Martin</au><au>Bohn, Erwin</au><au>Aepfelbacher, Martin</au><au>Autenrieth, Ingo B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells</atitle><jtitle>Inflammatory bowel diseases</jtitle><addtitle>Inflamm Bowel Dis</addtitle><date>2007-01</date><risdate>2007</risdate><volume>13</volume><issue>1</issue><spage>83</spage><epage>90</epage><pages>83-90</pages><issn>1078-0998</issn><eissn>1536-4844</eissn><abstract>Background: Lactobacilli represent a major component of the human microbiota. In this study we investigated whether and how Lactobacillus fermentum inhibits the proinflammatory responses of human epithelial cells on Yersinia enterocolitica infection.
Methods: Human epithelial cells were exposed to Y. enterocolitica pYV− or L. fermentum or to both strains, combinations of heat‐killed L. fermentum or supernatant of L. fermentum cultures and Y. enterocolitica. The modulation of Y. enterocolitica induced IL‐8 levels in the culture supernatants was determined and activation of Rac, p38, and NF‐κB was investigated.
Results: Exposure of human epithelial cells to L. fermentum does not induce NF‐κB activation and subsequent IL‐8 secretion in HeLa cells, whereas Y. enterocolitica induces NF‐κB activation and high levels of IL‐8. Viable L. fermentum, supernatant of L. fermentum cultures, but not heat‐killed L. fermentum, inhibited IL‐8 secretion of HeLa cells triggered by Y. enterocolitica. Lactobacillus fermentum‐exposed HeLa cells showed decreased Rac, p38, and NF‐κB activation after Y. enterocolitica infection. Treatment of L. fermentum supernatants with phospholipase C abolished the inhibitory effect, indicating that a secreted phospholipid mediates the antiinflammatory properties of L. fermentum. Adhesion to or invasion of Y. enterocolitica into epithelial cells was not altered by coincubation with L. fermentum.
Conclusion: Our results lead to the conclusion that L. fermentum inhibits the Y. enterocolitica‐induced IL‐8 production by a possibly secreted phospholipid of <10 kDa molecular weight. These data suggest that L. fermentum may have probiotic properties modulating intestinal inflammatory responses and might offer new therapeutic strategies in the treatment of intestinal inflammatory diseases.
(Inflamm Bowel Dis 2007;13:83–90)</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>17206643</pmid><doi>10.1002/ibd.20009</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Bacterial Adhesion Cell culture Cells, Cultured Culture Media, Conditioned Data processing Endopeptidase K - pharmacology Epithelial cells Epithelial Cells - metabolism Epithelial Cells - microbiology Glycerophospholipids - chemistry Glycerophospholipids - pharmacology HeLa Cells Humans IL‐8 Infection Inflammation Inflammation Mediators - metabolism Inflammatory bowel diseases Inflammatory diseases Interleukin 8 Interleukin-8 - secretion Intestine Iron Compounds - pharmacology Lactobacilli Lactobacillus Lactobacillus fermentum Lactobacillus fermentum - physiology Molecular weight NF- Kappa B protein NF-kappa B - metabolism p38 Mitogen-Activated Protein Kinases - metabolism Phospholipase C Phospholipids probiotics rac1 GTP-Binding Protein - metabolism Yersinia Yersinia enterocolitica Yersinia enterocolitica - pathogenicity Yersinia Infections - metabolism |
| title | Lactobacillus fermentum attenuates the proinflammatory effect of Yersinia enterocolitica on human epithelial cells |
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