Quartz Sand as an Adsorbent for Purification of Extracellular Glucose Oxidase from Penicillium funiculosum 46.1

A procedure for purification of extracellular glucose oxidase (GO, EC 1.1.3.4) from a filtrate of culture liquid (CLF) of the fungus Penicillium funiculosum 46.1 has been developed using alluvial quartz sand as an adsorbent. Modifying the sand by changing the charge and polarity did not lead to a si...

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Published in:Applied biochemistry and microbiology 2004-03, Vol.40 (2), p.151-157
Main Authors: Eryomin, AN, Drozhdenyuk, A P, Zhavnerko, G K, Semashko, T V, Mikhailova, R V
Format: Article
Language:English
Subjects:
Adsorption
Aluminum
Filtrate
Fractionation
Penicillium funiculosum
Quartz
Sand
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creator Eryomin, AN
Drozhdenyuk, A P
Zhavnerko, G K
Semashko, T V
Mikhailova, R V
description A procedure for purification of extracellular glucose oxidase (GO, EC 1.1.3.4) from a filtrate of culture liquid (CLF) of the fungus Penicillium funiculosum 46.1 has been developed using alluvial quartz sand as an adsorbent. Modifying the sand by changing the charge and polarity did not lead to a significant increase in its adsorption capacity towards GO. The effectiveness of sand and aluminum oxide used as adsorbents for GO isolation from CLF has been compared. Glucose oxidase isolated from CLF by adsorption on sand exhibited a greater catalytic activity than enzyme preparations obtained by column chromatography on CLF. Glucose oxidase from P. funiculosum 46.1 was adsorbed on sand more effectively than on aluminum oxide. It is concluded that sand may be used for fractionation of partially purified GO.
doi_str_mv 10.1023/B:ABIM.0000018918.34739.ae
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subjects Adsorption
Aluminum
Filtrate
Fractionation
Penicillium funiculosum
Quartz
Sand
title Quartz Sand as an Adsorbent for Purification of Extracellular Glucose Oxidase from Penicillium funiculosum 46.1
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