Exoproteinases of the Oomycete Phytophthora infestans

When grown in a medium containing heat-stable potato tuber proteins, the oomycete Phytophthora infestans (Mont.) de Bary produces a set of exoproteinases active at neutral and mildly basic pH values. These extracellular proteinases have been shown (by SDS-PAGE in the presence of gelatin) to include...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2004-03, Vol.40 (2), p.165-169
Main Authors: Gvozdeva, EL, Ievleva, E V, Gerasimova, NG, Ozeretskovskaya, O L, Valueva, T A
Format: Article
Language:English
Subjects:
Enzymes
Flowers & plants
Gelatin
Metalloproteinase
Molecular weight
Oomycetes
pH effects
Phytophthora infestans
Potatoes
Proteinase inhibitors
Serine proteinase
Solanum tuberosum
Subtilisin
Thermal stability
Trypsin
Tubers
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Summary:When grown in a medium containing heat-stable potato tuber proteins, the oomycete Phytophthora infestans (Mont.) de Bary produces a set of exoproteinases active at neutral and mildly basic pH values. These extracellular proteinases have been shown (by SDS-PAGE in the presence of gelatin) to include at least six components differing in molecular weight. Inhibitory analysis and studies of the effects of the enzymes on various synthetic substrates show that the culture liquid of P. infestans contains mainly serine proteinases (specific for trypsin and subtilisin) and metalloproteinases. Their activity is suppressed by proteinase-inhibiting proteins from potato tubers. It is suggested that exoproteinases of P. infestans may be the metabolic target for natural proteinase inhibitors from potato.
ISSN:0003-6838
1608-3024
DOI:10.1023/B:ABIM.0000018920.38585.60