Evaluation of a test kit for determination of serum immunoglobulin G concentration in foals

The accuracy of an immunoglobulin (Ig) G test kit for the semiquantitative measurement of IgG concentration was evaluated with serum from 88 foals. Failure of passive transfer (IgG < 400 mg/dl) was correctly identified in each of 34 samples, and partial failure of passive transfer (400 ≤ IgG <...

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Bibliographic Details
Published in:Journal of veterinary internal medicine 1988-10, Vol.2 (4), p.181-183
Main Authors: Bertone, J.J. (Colorado State University, Fort Collins, CO), Jones, R.L, Curtis, C.R
Format: Article
Language:English
Subjects:
Animals
BLOOD SERUM
ENSAYO
FOALS
Horses - immunology
Immunity, Maternally-Acquired
Immunization, Passive - veterinary
Immunodiffusion
IMMUNOENZYME TECHNIQUES
Immunoglobulin G - analysis
IMMUNOGLOBULINE
IMMUNOGLOBULINS
INMUNOGLOBULINA
POTRO
POULAIN
Reagent Kits, Diagnostic - veterinary
SERUM SANGUIN
SUERO SANGUINEO
TECHNIQUE IMMUNOENZYMATIQUE
TECNICAS INMUNOENZIMATICAS
TESTAGE
TESTING
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Summary:The accuracy of an immunoglobulin (Ig) G test kit for the semiquantitative measurement of IgG concentration was evaluated with serum from 88 foals. Failure of passive transfer (IgG < 400 mg/dl) was correctly identified in each of 34 samples, and partial failure of passive transfer (400 ≤ IgG < 800 mg/dl) was correctly identified in each of nine samples. Evidence of adequate passive transfer (IgG ≥ 800 mg/dl) was detected in 44 of 45 samples. One sample with 800 mg/dl or more of IgG was incorrectly classified as a partial failure of passive transfer (Kendall Tau ‐ b= .975). The high degree of accuracy, especially without any errors of overestimation of IgG concentrations, indicated that the IgG test kit should be a useful assay for rapidly determining the passive transfer status of foals. (Journal of Veterinary Internal Medicine 1988; 2:181–183)
ISSN:0891-6640
1939-1676
DOI:10.1111/j.1939-1676.1988.tb00314.x