Changes in the Activity of Superoxide Dismutase in Rauwolfia serpentina Benth. Callus Cultures Grown under Standard Conditions and Heat Shock

The rate of superoxide dismutase (SOD) accumulation inRauwolfia serpentinaBenth. cell culture under heat shock conditions (3 h, 45°C) decreased insignificantly (by 4%), whereas low positive temperature (24 h, 7°C) caused a drastic drop (by 48%). The observed decrease in the level of SOD activity res...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2004-01, Vol.40 (1), p.76-79
Main Author: Kirillova, N V
Format: Article
Language:English
Subjects:
Biosynthesis
Cell culture
Enzymes
Plant biology
Proteins
Temperature
Online Access:Get full text
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Summary:The rate of superoxide dismutase (SOD) accumulation inRauwolfia serpentinaBenth. cell culture under heat shock conditions (3 h, 45°C) decreased insignificantly (by 4%), whereas low positive temperature (24 h, 7°C) caused a drastic drop (by 48%). The observed decrease in the level of SOD activity resulted from a slowdown of the biosynthesis rate of the enzyme and a decrease in its concentration in the cultivated cells. In addition, a compensatory decrease in degradation of the active protein (K^sub d^) was observed at low positive temperatures and, consequently, an increase in its half-life (tt^sub 1/2^), compensating partially for a deficiency in de novo synthesized SOD molecules. The parameters studied were restored to normal after a 24-h adaptation of cells under standard temperature conditions.[PUBLICATION ABSTRACT]
ISSN:0003-6838
1608-3024
DOI:10.1023/B:ABIM.0000010358.97717.c8