Restriction Endonuclease Sst12I from a Strain of Streptomyces Recognizing the Nucleotide Sequence 5QUOTATION_MARK-CTGCAG-3QUOTATION_MARK

A new restriction endonuclease Sst12I belonging to type II and recognizing the sequence 5QUOTATION_MARK-CTGCAG-3QUOTATION_MARK was isolated from the bacterial strain Streptomycessp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2002-01, Vol.38 (1), p.20-22
Main Authors: Ushakova, T A, Puchkova, LI, Gutorov, V V, Kuvshinov, V N, Repin, V E
Format: Article
Language:English
Subjects:
Streptomyces
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Summary:A new restriction endonuclease Sst12I belonging to type II and recognizing the sequence 5QUOTATION_MARK-CTGCAG-3QUOTATION_MARK was isolated from the bacterial strain Streptomycessp. St-12. The enzyme hydrolyzes DNA between adenine and guanine residues; thus, it is a true isoschizomer of restrictase PstI. In contrast to PstI, the restriction endonuclease Sst12I hydrolyses DNA both at 37 and 55C and remains active after long-term storage.
ISSN:0003-6838
1608-3024
DOI:10.1023/A:1013288202420