Effects of cadmium on some haematological and biochemical characteristics of Oreochromis niloticus (Linnaeus, 1758) dietary supplemented with tomato paste and vitamin E

• Published in: Fish physiology and biochemistry 2011-03, Vol.37 (1), p.71-84
• Main Authors: Mekkawy, Imam A. A., Mahmoud, Usama M., Wassif, Ekbal T., Naguib, Mervat
• Format: Article
• Language: English
• Subjects: Alanine Transaminase - blood; Animal Anatomy; Animal Biochemistry; Animal Physiology; Animals; Antioxidants; Antioxidants - pharmacology; Aspartate Aminotransferases - blood; Behavior, Animal - drug effects; Biochemistry; Biomedical and Life Sciences; Blood; Blood Chemical Analysis; Blood Glucose - analysis; Blood Proteins - analysis; Cadmium; Cadmium - toxicity; Carotenoids; Carotenoids - pharmacology; Cichlids - blood; Cichlids - growth & development; Cichlids - physiology; Deoxyribonucleic acid; Diet; Dietary Supplements; DNA; DNA Fragmentation - drug effects; Erythrocyte Count; Erythrocytes; Free radicals; Freshwater & Marine Ecology; Heavy metals; Hematology; Hemoglobin; Histology; Life Sciences; Lipid peroxidation; Lipid Peroxidation - drug effects; Lipids; Lipids - blood; Liver; Liver - drug effects; Liver - metabolism; Lycopersicon esculentum; Morphology; Oreochromis niloticus; Peroxidation; Phytochemicals; Random Allocation; Tomatoes; Toxicity; Vitamin E; Vitamin E - pharmacology; Zoology
• ISSN: 0920-1742; 1573-5168
• DOI: 10.1007/s10695-010-9418-3

ABSTRACT The present study investigates the potential protective effects of tomato paste (9 mg/kg-lycopene) in comparison with vitamin E (50 mg/kg) against the impacts of cadmium (Cd) toxicity (4.64 mg/l: ¼ of 96 h LC50) on fishes Cd exposed for 15 and 30 days. Cd impacts were evaluated in terms of biological, haematological and biochemical characteristics. Cd significantly induced free radicals in serum and liver. The activities of aspartate aminotransferase and alanine aminotransferase in serum were significantly increased due to Cd. Treatment with Cd caused a significant increase in Lipid peroxidation and DNA fragmentation in liver tissue and serum glucose and total lipid. On the other hand, Cd significantly led to decline in serum total protein, blood haemoglobin, red blood cell count, haematocrit value, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration. Dietary supplementation with vitamin E and/or tomato paste to Cd-exposed fish declined significantly the increased lipid peroxidation and DNA fragmentation in liver tissue and the increased aspartate aminotransferase, alanine aminotransferase, glucose and total lipid in serum to the normal condition. This supplementation also significantly increased the declined serum total protein, blood haemoglobin, red blood cell count, haematocrit value, mean corpuscular volume, mean corpuscular haemoglobin and mean corpuscular haemoglobin concentration to the normal state. Cd impacts and tomato paste/or vitamin E supplementations did not reflected on the condition factor of the fish. These findings demonstrated the beneficial diet supplementation of tomato paste phytonutrients and vitamin E in counteracting the harmful effects of Cd on the characters investigated.