Role of transamination in the mobilization of respiratory substrates in germinating seeds of castor oil plants

The metabolism of 1,4-¹⁴C-succinate and 2,3-¹⁴C-succinate and the activity of succinic semialdehyde dehydrogenase (EC 1.2.1.16) were studied in germinating seeds of castor oil plants (Ricinus communis L.). Succinate metabolism involved succinate dehydrogenase and was sensitive to metabolites of the...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2007-05, Vol.43 (3), p.341-346
Main Authors: Popov, V. N, Eprintsev, A. T, Fedorin, D. N, Fomenko, O. Yu, Igamberdiev, A. U
Format: Article
Language:English
Subjects:
Amino acids
Castor oil
Dehydrogenase
endosperm
gamma-aminobutyric acid
germination
metabolism
Metabolites
oil crops
Ricinus communis
Seeds
succinate dehydrogenase (quinone)
succinic acid
transamination
tricarboxylic acid cycle
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Summary:The metabolism of 1,4-¹⁴C-succinate and 2,3-¹⁴C-succinate and the activity of succinic semialdehyde dehydrogenase (EC 1.2.1.16) were studied in germinating seeds of castor oil plants (Ricinus communis L.). Succinate metabolism involved succinate dehydrogenase and was sensitive to metabolites of the γ-aminobutyric acid shunt. Considerable accumulation of the label in amino acids reflected the progression of transamination reactions. Succinic semialdehyde dehydrogenase was purified from the endosperm of castor oil plants. Kinetic characteristics of the enzyme were evaluated. Our study indicates that the mobilization of respiratory substrates during germination of castor oil plants is related to active transamination of ketoacids in the Krebs cycle and involves the γ-aminobutyric acid shunt.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683807030179