Neuroprotective Effects of 3α-Acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide Against Dopamine-Induced Apoptosis in the Human Neuroblastoma SH-SY5Y Cell Line

Dopamine (DA), as a neurotoxin, can elicit severe Parkinson’s disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. We examined the inhibitory effects of 3α-acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide (AETO), purified from the leaves of Lau...

Full description

Saved in:
Bibliographic Details
Published in:Neurochemical research 2011-11, Vol.36 (11), p.1991-2001
Main Authors: Koo, Uk, Nam, Kung-Woo, Ham, Ahrom, Lyu, Dahyun, Kim, Bora, Lee, Sung-Jin, Kim, Kyeong Ho, Oh, Ki-Bong, Mar, Woongchon, Shin, Jongheon
Format: Article
Language:English
Subjects:
alpha-Synuclein - biosynthesis
Antioxidants - therapeutic use
Apoptosis
Apoptosis - drug effects
Bcl-2 protein
Biochemistry
Biomedical and Life Sciences
Biomedicine
Caspase 3 - metabolism
Caspase-3
Cell Biology
Cell Line, Tumor
Dopamine
Dopamine - pharmacology
Flow cytometry
Humans
Laurus - chemistry
Laurus nobilis
Leaves
Monophenol monooxygenase
Monophenol Monooxygenase - antagonists & inhibitors
Movement disorders
Neuroblastoma
Neurochemistry
Neurodegenerative diseases
Neurology
Neuroprotection
Neuroprotective Agents - pharmacology
Neurosciences
Neurotoxins
Original Paper
p53 protein
Parkinson's disease
Proto-Oncogene Proteins c-bcl-2 - metabolism
quinoproteins
Reactive oxygen species
Reactive Oxygen Species - metabolism
Sesquiterpenes, Eudesmane - therapeutic use
Synuclein
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Dopamine (DA), as a neurotoxin, can elicit severe Parkinson’s disease-like syndrome by elevating intracellular reactive oxygen species (ROS) levels and apoptotic activity. We examined the inhibitory effects of 3α-acetoxyeudesma-1,4(15),11(13)-trien-12,6α-olide (AETO), purified from the leaves of Laurus nobilis L., on DA-induced apoptosis and α-synuclein (α-syn) formation in dopaminergic SH-SY5Y cells. AETO decreased the active form of caspase-3 and the levels of p53, which were accompanied by increased levels of Bcl-2 in a dose-dependent manner. Flow cytometric and Western blot analysis showed that AETO significantly inhibited DA-induced apoptosis along with suppression of intracellular tyrosinase activity, ROS generation, quinoprotein, and α-syn formation ( P  
ISSN:0364-3190
1573-6903
DOI:10.1007/s11064-011-0523-1