Purinergic Membrane Receptors as Targets for the Effect of Purotoxin 1, a Component of Venom of Spiders from the Geolycosa Genus

We examined effects of purotoxin 1 (PT1), a component of the venom of Geolycosa spiders, on a few voltageand ligand-operated ion channels present in the plasma membrane of sensory neurons from the rat dorsal root ganglia (DRGs). Purotoxin 1 in a 100 nM concentration evoked no changes in ion currents...

Full description

Saved in:
Bibliographic Details
Published in:Neurophysiology (New York) 2011-05, Vol.42 (6), p.387-391
Main Authors: Savchenko, G. A., Volkova, T. M., Vasilevskii, A. A., Korol’kova, Yu. V., Grishin, E. V., Boichouk, Ya. A., Krishtal, O. A.
Format: Article
Language:English
Subjects:
Araneae
Biomedical and Life Sciences
Biomedicine
Calcium channels
Geolycosa
Identification and classification
Neurobiology
Neurons
Neurosciences
Plasma
Spiders
Venom
Zoology
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We examined effects of purotoxin 1 (PT1), a component of the venom of Geolycosa spiders, on a few voltageand ligand-operated ion channels present in the plasma membrane of sensory neurons from the rat dorsal root ganglia (DRGs). Purotoxin 1 in a 100 nM concentration evoked no changes in ion currents through voltage-operated sodium, potassium, and calcium channels in the membranes of isolated sensory neurons. This agent was also found to be ineffective with respect to capsaicin-sensitive receptor-channel complexes (TRPV1). Testing of the effects of PT1 on purinergic receptor-channel complexes P2X3, P2X2, and P2X2/3 showed that this toxin is a highly selective blocker of exclusively P2X3 receptors. The selectivity of action of PT1 demonstrated in our experiments shows that it is a unique agent, which opens up new prospects in the studies of structural/functional peculiarities of receptor-channel complexes P2X3 as a peripheral link of the nociception system.
ISSN:0090-2977
1573-9007
DOI:10.1007/s11062-011-9173-9