Isolation of Cell Membranes from Saccharomyces cerevisiae for Evaluation of Their Protein Composition

We describe a simple method for the isolation of membrane fractions from Saccharomyces cerevisiae yeasts containing a complex of plasma membranes and cell walls. The method is based on cell disruption on an INBI flow disintegrator. This device spares subcellular structures, which simplifies the isol...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2004-05, Vol.40 (3), p.296-299
Main Authors: Aliverdieva, DA, Malygin, A G, Lagutina, L S, Sholtz, K F
Format: Article
Language:English
Subjects:
Cell disruption
Cell membranes
Cell walls
Cells
Disintegrators
Gel electrophoresis
Membranes
Plasma membranes
Protein composition
Proteins
Saccharomyces cerevisiae
Yeasts
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Description
Summary:We describe a simple method for the isolation of membrane fractions from Saccharomyces cerevisiae yeasts containing a complex of plasma membranes and cell walls. The method is based on cell disruption on an INBI flow disintegrator. This device spares subcellular structures, which simplifies the isolation of cell membranes. The membrane fraction obtained by this method was suitable for studies of the protein composition of these structures by means of two-dimensional gel electrophoresis.
ISSN:0003-6838
1608-3024
DOI:10.1023/B:ABIM.0000025955.26547.04