Plastid transformation in eggplant (Solanum melongena L.)

We have developed a method for plastid transformation in eggplant ( Solanum melongena L.), a solanaceous plant species. Plastid transformation in eggplant was achieved by bombardment of green stem segments with pPRV111A plastid expression vector carrying the aadA gene encoding aminoglycoside 3′′-ade...

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Bibliographic Details
Published in:Transgenic research 2010-02, Vol.19 (1), p.113-119
Main Authors: Singh, A. K., Verma, S. S., Bansal, K. C.
Format: Article
Language:English
Subjects:
aadA gene
Animal Genetics and Genomics
Anti-Bacterial Agents - pharmacology
Biological and medical sciences
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biotechnology
Brief Communication
Crops, Agricultural - genetics
Drug Resistance, Microbial - genetics
Explants
Expression vectors
Fundamental and applied biological sciences. Psychology
Genetic Engineering
Genetic Engineering - methods
Genetic technics
Integration
Life Sciences
Methods. Procedures. Technologies
Molecular Medicine
Pharmaceuticals
Plant Genetics and Genomics
Plants, Genetically Modified
Plasmids
Plastids
Plastids - genetics
plastomes
Polymerase chain reaction
Probes
rRNA 16S
Solanum melongena
Solanum melongena - genetics
Southern blotting
Spectinomycin - pharmacology
Transformation
Transformation, Genetic - physiology
Transgenic animals and transgenic plants
Transgenics
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Summary:We have developed a method for plastid transformation in eggplant ( Solanum melongena L.), a solanaceous plant species. Plastid transformation in eggplant was achieved by bombardment of green stem segments with pPRV111A plastid expression vector carrying the aadA gene encoding aminoglycoside 3′′-adenylyltransferase. Biolistic delivery of the pPRV111A plasmid yielded transplastomic plants at a frequency of two per 21 bombarded plates containing 25 stem explants each. Integration of the aadA gene in the plastome was verified by PCR analysis and also by Southern blotting using 16S rDNA (targeting sequence) and the aadA gene as a probe. Transplastomic expression of the aadA gene was verified by RT-PCR. The development of transplastomic technology in eggplant may open up exciting possibilities for novel gene introduction and expression in the engineered plastome for agronomic or pharmaceutical traits.
ISSN:0962-8819
1573-9368
DOI:10.1007/s11248-009-9290-z