Influence of the KDEL signal, DMSO and mannitol on the production of the recombinant antibody 14D9 by long-term Nicotiana tabacum cell suspension culture

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2010-12, Vol.103 (3), p.307-314
• Main Authors: López, J., Lencina, F., Petruccelli, S., Marconi, P., Alvarez, M. A.
• Format: Article
• Language: English
• Subjects: Antibodies; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Catalysis; Cell culture; Dimethyl sulfoxide; Endoplasmic reticulum; Fundamental and applied biological sciences. Psychology; Life Sciences; Mannitol; Nicotiana tabacum; Original Paper; Osmotic stress; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Suspension culture
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-010-9780-y

We have established two transgenic cell suspension culture lines of Nicotiana tabacum that express the catalytic antibody 14D9 as a secretory product (sec-Ab) or as a KDEL-tagged product in the endoplasmic reticulum (Ab-KDEL), respectively. After 3 years of culture, the performance improved to a production level of 0.15 ± 0.03 μg ml −1 on the seventh day of culture for the sec-Ab line and 0.48 ± 0.05 μg ml −1 on the third day for Ab-KDEL line. Analysis of the effect of osmotic stress using mannitol (90 g l −1 ) as an osmolite revealed that there was a 12-fold increase in antibody yield (1.96 ± 0.20 μg ml −1 ) on the seventh day of culture in line sec-Ab and a fivefold increase (2.31 ± 0.18 μg ml −1 ) on the seventh day for line Ab-KDEL. The concentration of the antibody in the culture medium was not significant. Dimethyl sulfoxide used as a permeabilizing agent was not effective in increasing 14D9 yield, but it did cause distinctive cell damage at all concentrations tested.