Changes in metabolic substrates during early development in anchoveta Engraulis ringens (Jenyns 1842) in the Humboldt Current

• Published in: Marine biology 2010-05, Vol.157 (5), p.1137-1149
• Main Authors: Krautz, M. C, Vásquez, S, Castro, L. R, González, M, Llanos-Rivera, A, Pantoja, S
• Format: Article
• Language: English
• Subjects: Agnatha. Pisces; Amino acids; Anchovies; Animal and plant ecology; Animal, plant and microbial ecology; Aquaculture; Biological and medical sciences; Biological research; Biology, Experimental; Biomedical and Life Sciences; Chemical properties; Eggs; Engraulis ringens; Fish; Fishes; Freshwater & Marine Ecology; Fundamental and applied biological sciences. Psychology; Genetic aspects; Hatching; Larvae; Larval development; Life Sciences; Lipids; Marine; Marine & Freshwater Sciences; Marine biology; Microbiology; Oceanography; Offspring; Original Paper; Proteins; Sea water ecosystems; Spawning; Synecology; Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution; Zoology
• ISSN: 0025-3162; 1432-1793
• DOI: 10.1007/s00227-010-1395-7

We assessed the ontogenetic changes in protein content and free amino acids (FAA) in eggs and early larvae of Engraulis ringens (anchoveta) off central Chile on different dates during the spawning season. On all sampling dates, a reduction in embryonic yolk-sac volume, proteins and FAA concentrations occurred during development. Protein electrophoresis (SDS-PAGE) of eggs and larvae showed at least 22 protein bands: 11 were consumed early and not detected after hatching. The proportion of essential FAA (EFAA) was higher than the proportion of non-essential FAA (NEFAA) in early eggs and in 7 day-old larvae (82.5-73% EFAA respectively). During egg development, the FAA pool was dominated by leucine, alanine and lysine, three amino acids contributing 35-44% of the total FAA in eggs. During larval development, histidine was the most abundant FAA. In July, total FAA constituted 13-18% of the egg dry weight. A similar proportion (45-51%) occurred in July between protein plus FAA and total lipids. The differences in egg size during the spawning season along with variability in batch composition suggests that the female spawning condition is a major factor determining egg quality and early offspring success.