Direct contact between Pseudo-nitzschia multiseries and bacteria is necessary for the diatom to produce a high level of domoic acid

Pseudo-nitzschia multiseries loses most of its ability to produce domoic acid when it is cultured axenically. However, domoic acid production recovers when bacteria from the original culture are reintroduced to axenic cultures, indicating a bacterial association with domoic acid production in this s...

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Bibliographic Details
Published in:Fisheries science 2009-05, Vol.75 (3), p.771-776
Main Authors: Kobayashi, K.(Kitasato Univ., Ofunato, Iwate (Japan). School of Marine Biosciences), Takata, Y, Kodama, M
Format: Article
Language:English
Subjects:
Acid production
Acids
BACILLARIOPHYCEAE
BACTERIA
Biomedical and Life Sciences
Cell culture
CULTURE TECHNIQUES
ENVENENAMIENTO
Fish & Wildlife Biology & Management
FITOPLANCTON
FITOTOXICIDAD
Food Science
Freshwater & Marine Ecology
INTOXICATION
Life Sciences
NITZSCHIACEAE
Original Article
PHYTOPLANCTON
PHYTOPLANKTON
PHYTOTOXICITE
PHYTOTOXICITY
Plankton
POISONING
Pseudo-nitzschia multiseries
TECHNIQUE DE CULTURE
TECNICAS DE CULTIVO
TOXINAS
TOXINE
TOXINS
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Summary:Pseudo-nitzschia multiseries loses most of its ability to produce domoic acid when it is cultured axenically. However, domoic acid production recovers when bacteria from the original culture are reintroduced to axenic cultures, indicating a bacterial association with domoic acid production in this species. In order to determine the role of bacteria in domoic acid production, an axenic strain prepared from a nonaxenic strain of P. multiseries was inoculated into media in cellophane tubes, which were then placed in a bottle containing the original nonaxenic culture. Both strains showed characteristic domoic acid production similar to that previously observed. Domoic acid level in the cells of the axenic strain in the tubes was much smaller than that of the cells outside the tubes. These results show that direct contact between living bacteria and P. multiseries is necessary for producing high levels of domoic acid in P. multiseries.
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-009-0081-5