A chemically defined production process for highly attenuated poxviruses

Highly attenuated poxviruses are promising vectors for protective and therapeutic vaccines. These vectors do not replicate in human cells and can therefore be safely given even to immunocompromised recipients. They can accomodate very large inserts and provide strong stimulation of the immune system...

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Bibliographic Details
Published in:Biologicals 2011, Vol.39 (1), p.50-58
Main Authors: Jordan, Ingo, Northoff, Stefan, Thiele, Michael, Hartmann, Stefan, Horn, Deborah, Höwing, Kristin, Bernhardt, Holger, Oehmke, Stefanie, von Horsten, Henning, Rebeski, Dierk, Hinrichsen, Lars, Zelnik, Vladimir, Mueller, Wiebke, Sandig, Volker
Format: Article
Language:English
Subjects:
AGE1.CR
ALVAC
Animals
antigens
Bioreactors
Canarypox virus - genetics
Canarypox virus - immunology
Cell Line
Cell Proliferation
CHO Cells
CR.pIX
Cricetinae
Cricetulus
fowl pox
Fowlpox
Fowlpox virus - genetics
Fowlpox virus - immunology
Genetic Vectors - genetics
Genetic Vectors - immunology
Humans
immune system
MVA
Poxviridae
Poxviridae - genetics
Poxviridae - immunology
Vaccine production
vaccines
Vaccines, Attenuated - immunology
Vaccinia virus - genetics
Vaccinia virus - immunology
Viral Vaccines - immunology
Virus Replication - genetics
viruses
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Description
Summary:Highly attenuated poxviruses are promising vectors for protective and therapeutic vaccines. These vectors do not replicate in human cells and can therefore be safely given even to immunocompromised recipients. They can accomodate very large inserts and provide strong stimulation of the immune system against the vectored antigen. Disadvantages include that very high numbers of infectious units are required per dose for full efficacy. Because they are difficult to produce, improved cellular substrates and processes are urgently needed to facilitate programs intended to reach a large number of vaccinees. We have developed a fully scalable and very efficient chemically-defined production process for modified vaccinia Ankara (MVA), canarypox (CNPV, strain ALVAC) and fowlpox viruses (FPV) based on a continuous cell line.
ISSN:1045-1056
1095-8320
DOI:10.1016/j.biologicals.2010.11.005