An Insoluble Colored Substrate for Dextranase Assay

An assay of dextranase (EC 3.2.1.11) was developed by using Sephadex G-200 coupled with Remazol Brilliant Blue (RBB) as an insoluble substrate. The assay procedure included incubation of the suspension of the colored substrate in a buffer containing the enzyme under study, removal of a residual inso...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2002-01, Vol.38 (1), p.89-93
Main Authors: Khalikova, E F, Usanov, N G
Format: Article
Language:English
Subjects:
Bacteria
Bioassays
Enzymes
Microbiology
Substrates
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Summary:An assay of dextranase (EC 3.2.1.11) was developed by using Sephadex G-200 coupled with Remazol Brilliant Blue (RBB) as an insoluble substrate. The assay procedure included incubation of the suspension of the colored substrate in a buffer containing the enzyme under study, removal of a residual insoluble substrate, and measurement of the absorbance of supernatant fluid containing colored soluble hydrolysis products at 595 nm. The procedure was examined in the screening of dextranase-forming bacilli from the microbial collection of the Institute of Biology, Ufa Research Center, RAS.[PUBLICATION ABSTRACT]
ISSN:0003-6838
1608-3024
DOI:10.1023/A:1013217108781