Suppression of embryonic lung branching morphogenesis by antisense oligonucleotides against HOM/C homeobox factors

The role of HOM/C homeobox genes on rat embryonic lung branching morphogenesis was investigated using the lung bud explant culture system in an air/liquid interface. Knock down of homeobox b3 and b4 expression by antisense oligonucleotide treatment repressed airway branch formation, while antisense...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2010-09, Vol.46 (8), p.664-672
Main Authors: Yoshimi, Tatsuya, Hashimoto, Fumiko, Takahashi, Shigeru, Takahashi, Yuji
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Antibodies
Antisense oligonucleotides
Biomedical and Life Sciences
CELL AND TISSUE MODELS
Cell Biology
Cell Culture
Collagen Type III - genetics
Collagens
Developmental Biology
Epithelial cells
Fibroblast Growth Factor 10 - genetics
Genes
Homeobox genes
Homeodomain Proteins - genetics
Homeodomain Proteins - physiology
Life Sciences
Lung - embryology
Lung - metabolism
Lungs
Messenger RNA
Morphogenesis
Oligonucleotides
Oligonucleotides, Antisense - genetics
Rats
Rats, Sprague-Dawley
Stem Cells
Up-Regulation
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Summary:The role of HOM/C homeobox genes on rat embryonic lung branching morphogenesis was investigated using the lung bud explant culture system in an air/liquid interface. Knock down of homeobox b3 and b4 expression by antisense oligonucleotide treatment repressed airway branch formation, while antisense oligonucleotide against homeobox a3 showed no effect. Addition of antisense Hoxb3 oligonucleotide resulted in upregulation of collagen type III mRNA and fibroblast growth factor 10 mRNA, while that of the T-box regulatory factor-4 was decreased. Consequently, expression of Clara cell-specific secretory protein was decreased. These results suggest a critical role for homeobox b3 and b4 genes in lung airway branching morphogenesis.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-010-9322-2