Improvement of isolated microspore culture of pepper (Capsicum annuum L.) via co-culture with ovary tissues of pepper or wheat

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2009-06, Vol.97 (3), p.285-293
• Main Authors: Lantos, Csaba, Juhász, Anikó Gémes, Somogyi, György, Ötvös, Krisztina, Vági, Pál, Mihály, Róbert, Kristóf, Zoltán, Somogyi, Norbert, Pauk, János
• Format: Article
• Language: English
• Subjects: Anthers; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Capsicum annuum; coculture; Developmental stages; Fundamental and applied biological sciences. Psychology; genotype; Genotypes; gynoecium; leaves; Life Sciences; Microspores; Original Paper; Ovaries; Peppers; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Plantlets; spores; tissue culture; Triticum aestivum; Vegetables; Wheat
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-009-9527-9

The influence of the developmental stage of microspores on establishing isolated microspore cultures of three Hungarian ('Szegedi 80', 'Szegedi 178', and 'Remény') and three Spanish ('Jeromin', 'Jariza', and 'Jaranda') pepper genotypes was investigated. Donor anthers containing 80% uninucleated and 20% binucleated microspores yielded the highest frequency of successful microspore cultures. Co-cultures with wheat, line 'CY-45', ovaries exhibited enhanced frequency of embryoid production than those with pepper ovaries. Differences in efficiency of isolated pepper microspore culture establishment were observed among different pepper genotypes. Green plantlets were regenerated from microspore-derived embryoids, but some were exhibited abnormal growth habits, such as leaf rosetting. A total of seven fertile microspore-derived plants were obtained, including three 'Jariza', three 'Jaranda', and a single 'Szegedi 80' plant.