High-resolution mapping of the Brassica napus Rfp restorer locus using Arabidopsis-derived molecular markers

The two forms of cytoplasmic male sterility (CMS) native to the oilseed rape or canola species Brassica napus, nap and pol, have novel features that may provide insight into the molecular mechanisms through which CMS/nuclear restorer systems evolve. One such feature is the finding that the distinct...

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Bibliographic Details
Published in:Theoretical and applied genetics 2010-02, Vol.120 (4), p.843-851
Main Authors: Formanová, Nataša, Stollar, Rachel, Geddy, Rachel, Mahé, Laetitia, Laforest, Martin, Landry, Benoit S, Brown, Gregory G
Format: Article
Language:English
Subjects:
Agriculture
Arabidopsis
Arabidopsis - genetics
Arabidopsis thaliana
Biochemistry
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Brassica napus
Brassica napus - genetics
Brassica napus - physiology
Chromosome Mapping
Classical genetics, quantitative genetics, hybrids
Fundamental and applied biological sciences. Psychology
Genes
Genes, Plant
Genetic Markers
Genetics of eukaryotes. Biological and molecular evolution
Genomes
Genomics
Life Sciences
Original Paper
Plant Biochemistry
Plant Breeding/Biotechnology
Plant Genetics and Genomics
Polymorphism, Restriction Fragment Length
Pteridophyta, spermatophyta
Vegetals
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Summary:The two forms of cytoplasmic male sterility (CMS) native to the oilseed rape or canola species Brassica napus, nap and pol, have novel features that may provide insight into the molecular mechanisms through which CMS/nuclear restorer systems evolve. One such feature is the finding that the distinct nuclear restorer genes for the two systems represent different alleles or haplotypes of the same nuclear locus. Improved understanding of how these systems have evolved will require molecular cloning and characterization of this novel locus. We have employed an approach that exploits the regional co-linearity between the Arabidopsis and Brassica genomes to construct a high-resolution genetic map of the nuclear restorer for the pol system, Rfp. Specifically, Arabidopsis-derived sequences have been used as a set of ordered RFLP probes to localize Rfp to a region of the B. napus genome equivalent to a 115 kb interval on Arabidopsis chromosome 1. Based on the known relationship of physical distances between orthologous segments of Arabidopsis and Brassica chromosomes, it is anticipated that the B. napus restorer locus is now mapped to sufficient resolution to permit its isolation and characterization.
ISSN:0040-5752
1432-2242
DOI:10.1007/s00122-009-1215-y