Characterisation of a cyclin-dependent kinase (CDKA) gene expressed during somatic embryogenesis of coconut palm

The CDKA gene is linked to the cellular control. This gene was isolated from coconut palm (Cocos nucifera L) and a detailed expression analysis was done during somatic embryogenesis. Analysis of the deduced amino acid sequence showed the most important residues to be conserved. The highest homology...

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Published in:Plant cell, tissue and organ culture tissue and organ culture, 2010-08, Vol.102 (2), p.251-258
Main Authors: Montero-Cortés, Mayra, Rodríguez-Paredes, Francisco, Burgeff, Caroline, Pérez-Nuñez, Teresa, Córdova, Iván, Oropeza, Carlos, Verdeil, Jean-Luc, Sáenz, Luis
Format: Article
Language:English
Subjects:
Amino acid sequence
amino acid sequences
Amino acids
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Callus
callus formation
CdkA gene
coconuts
Cocos nucifera
Conserved sequence
Cyclin-dependent kinase
Cyclin-dependent kinases
Embryonic growth stage
Embryos
Fundamental and applied biological sciences. Psychology
Gene expression
gene expression regulation
germination
Homology
Hybridization
Kinases
Life Sciences
Localization
meristems
messenger RNA
molecular sequence data
nucleic acid hybridization
nucleotide sequences
nut crops
Picea abies
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
protein structure
protein synthesis
Research Note
sequence analysis
Somatic embryogenesis
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Summary:The CDKA gene is linked to the cellular control. This gene was isolated from coconut palm (Cocos nucifera L) and a detailed expression analysis was done during somatic embryogenesis. Analysis of the deduced amino acid sequence showed the most important residues to be conserved. The highest homology was with Picea abies (96% similarity). Expression of the putative CnCDKA gene steadily increased during embryogenic callus formation phase when embryogenic competence is attained. In situ hybridization specified the localization of the transcripts as being mainly in a few cell layers within the meristematic centres in embryogenic calli at 90 day cultures. Analysis of CnCDKA expression at different somatic embryo formation stages showed that the expression was decreased progressively with the lowest expression in germinated somatic embryos.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-010-9714-8