Development and application of photoautotrophic micropropagation plant system

Research has revealed that most chlorophyllous explants/plants in vitro have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is not due to poor photosynthetic ability, but to the low CO 2 concentr...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2011-05, Vol.105 (2), p.149-158
Main Authors: Xiao, Yulan, Niu, Genhua, Kozai, Toyoki
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Carbon dioxide
Environmental conditions
Environmental effects
Explants
Gelation
Life Sciences
Light intensity
Luminous intensity
Micropropagation
Photosynthesis
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Plant species
Plantlets
Porosity
Porous materials
Relative humidity
Review
Sugar
Vessels
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Summary:Research has revealed that most chlorophyllous explants/plants in vitro have the ability to grow photoautotrophically (without sugar in the culture medium), and that the low or negative net photosynthetic rate of plants in vitro is not due to poor photosynthetic ability, but to the low CO 2 concentration in the air-tight culture vessel during the photoperiod. Moreover, numerous studies have been conducted on improving the in vitro environment and investigating its effects on growth and development of cultures/plantlets on nearly 50 species since the concept of photoautotrophic micropropagation was developed more than two decades ago. These studies indicate that the photoautotrophic growth in vitro of many plant species can be significantly promoted by increasing the CO 2 concentration and light intensity in the vessel, by decreasing the relative humidity in the vessel, and by using a fibrous or porous supporting material with high air porosity instead of gelling agents such as agar. This paper reviews the development and characteristics of photoautotrophic micropropagation systems and the effects of environmental conditions on the growth and development of the plantlets. The commercial applications and the perspective of photoautotrophic micropropagation systems are discussed.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-010-9863-9