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Application of Delta alpha -glucosidase activity and drug susceptibility tests to epidemiological studies on the fish pathogen Nocardia seriolae
This study determined the minimum inhibitory concentrations (MICs) for oxytetracycline hydrochloride (OTC) and erythromycin (Em), along with the Delta alpha -glucosidase ( Delta alpha -glu) activities in 110 Nocardia seriolae strains isolated in Miyazaki and Kagoshima prefectures in 2008--2009. The...
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Published in: | Fisheries science 2010-02, Vol.74 (6), p.113-118 |
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container_title | Fisheries science |
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creator | Ismail, Tamer Fawzy Takeshita, Akira Umeda, Naoko Itami, Toshiaki Yoshida, Terutoyo |
description | This study determined the minimum inhibitory concentrations (MICs) for oxytetracycline hydrochloride (OTC) and erythromycin (Em), along with the Delta alpha -glucosidase ( Delta alpha -glu) activities in 110 Nocardia seriolae strains isolated in Miyazaki and Kagoshima prefectures in 2008--2009. The strains were examined for the presence of the tet(K), tet(L), tet(M), tet(O), tet(S), erm(A), erm(B), mph(A), mef(A), and msr(D) genes. All the Delta alpha -glu-positive strains (n = 15) were OTC resistant and Em sensitive, with MICs of 32--64 and 128 Delta is a subset of g/ml. The MICs for Em in the remaining 2 Delta alpha -glu-negative strains were |
doi_str_mv | 10.1007/s12562-010-0302-y |
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The strains were examined for the presence of the tet(K), tet(L), tet(M), tet(O), tet(S), erm(A), erm(B), mph(A), mef(A), and msr(D) genes. All the Delta alpha -glu-positive strains (n = 15) were OTC resistant and Em sensitive, with MICs of 32--64 and <0.125 Delta is a subset of g/ml, respectively. All the Delta alpha -glu-negative strains (n = 95) were OTC sensitive, with MICs of 2--4 Delta is a subset of g/ml, and most of them (93 of 95) were Em resistant, with MICs of >128 Delta is a subset of g/ml. The MICs for Em in the remaining 2 Delta alpha -glu-negative strains were <0.125 Delta is a subset of g/ml. The 15 OTC-resistant strains possessed the tet(K) and/or tet(L) gene(s), and all of the 93 Em-resistant strains possessed both the mef(A) and msr(D) genes. The relationship between Delta alpha -glu activity and drug sensitivity of the N. seriolae strains may explain the difference in prevalence of each phenotype. 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language | eng |
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source | ABI/INFORM Global; Springer Nature |
subjects | Nocardia seriolae |
title | Application of Delta alpha -glucosidase activity and drug susceptibility tests to epidemiological studies on the fish pathogen Nocardia seriolae |
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