Classical swine fever virus in plasma and peripheral blood mononuclear cells of acutely infected swine

The distribution of classical swine fever virus (CSFV) in plasma, monocytes, T and B lymphocytes in peripheral blood was monitored during experimentally induced acute classical swine fever infection in piglets. Six piglets were infected with 103.8 TCID50 of virus and blood samples taken up to 18 day...

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Bibliographic Details
Published in:Journal of veterinary medicine. Series B 1999-11, Vol.46 (9), p.585-593
Main Authors: Gisler, A.C.F, Nardi, N.B, Nonnig, R.B, Oliveira, L.G, Roehe, P.M
Format: Article
Language:English
Subjects:
Acute Disease
Animals
Antibodies, Viral - biosynthesis
B-lymphocytes
B-Lymphocytes - immunology
B-Lymphocytes - virology
blood plasma
Classical Swine Fever - blood
Classical Swine Fever - immunology
Classical Swine Fever - virology
Classical swine fever virus
Classical swine fever virus - isolation & purification
disease course
experimental infections
Immunoenzyme Techniques - veterinary
immunoglobulin M
Leukocyte Count - veterinary
leukocytes
Mice
monocytes
Monocytes - immunology
Monocytes - virology
piglets
Swine
symptoms
T-lymphocytes
T-Lymphocytes - immunology
T-Lymphocytes - virology
Viremia - blood
Viremia - immunology
Viremia - virology
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Summary:The distribution of classical swine fever virus (CSFV) in plasma, monocytes, T and B lymphocytes in peripheral blood was monitored during experimentally induced acute classical swine fever infection in piglets. Six piglets were infected with 103.8 TCID50 of virus and blood samples taken up to 18 days post‐inoculation (p.i.). Infectious virus was detected in monocytes, T and B lymphocytes to similar titres in five of the six infected piglets. Infectious virus was detected earlier in plasma than in any of the mononuclear cell subpopulations. No significant difference was observed in the period of time in which virus could be isolated from the three cell subpopulations. While a progressive lymphopenia developed, a marked B cell depletion was observed. However, B cells were apparently replaced by non‐IgM‐bearing mononuclear cells, as the proportion ‘total lymphocyte/total leucocytes’ remained unaltered throughout the experiment. Virus titres in plasma and peripheral blood mononuclear cells showed a tendency to increase as the disease progressed to its outcome.
ISSN:0931-1793
0514-7166
1439-0450
DOI:10.1046/j.1439-0450.1999.00286.x