Antioxidative and Radiation Modulating Properties of Guanosine-5′-Monophosphate

• Published in: Nucleosides, nucleotides & nucleic acids nucleotides & nucleic acids, 2010-10, Vol.29 (10), p.786-799
• Main Authors: Asadullina, N. R., Usacheva, A. M., Smirnova, V. S., Gudkov, S. V.
• Format: Article
• Language: English
• Subjects: 8-oxoguanine; Animals; antioxidant; Antioxidants - administration & dosage; Antioxidants - metabolism; Antioxidants - pharmacology; Blood Cell Count; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; Bone Marrow Cells - radiation effects; Cattle; Deoxyguanosine - analogs & derivatives; Deoxyguanosine - metabolism; DNA - metabolism; Guanosine Monophosphate - administration & dosage; Guanosine Monophosphate - metabolism; Guanosine Monophosphate - pharmacology; guanosine-5′-monophosphate; long-lived radicals; Male; Mice; Radiation-Protective Agents - administration & dosage; Radiation-Protective Agents - metabolism; Radiation-Protective Agents - pharmacology; reactive oxygen species; Reactive Oxygen Species - metabolism; Serum Albumin, Bovine - metabolism; Survival Analysis; x-ray irradiation; X-Rays
• ISSN: 1525-7770; 1532-2335
• DOI: 10.1080/15257770.2010.518576

Employing enhanced chemiluminescence in luminol-p-iodophenol peroxidase system and coumarine-3-carboxylic acid, it was shown that guanosine-5′-monophosphate (GMP) appreciably reduces formation of H 2 O 2 and hydroxyl radicals induced by x-ray irradiation. Using immunoenzyme assay, we revealed that GMP lowered 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) formation in DNA in vitro after irradiation. The results of survival test have shown that mice being injected intraperitoneally with GMP after irradiation with a dose of 7 Gy had better survival rate than the control mice. GMP reduced leucopoenia and thrombocytopenia in irradiated mice. Obtained results give premises that GMP may be promising therapeutic agent for treatment of radiation injuries.