Preparation and physical properties of a novel biocompatible porcine corneal acellularized matrix

This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake...

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Bibliographic Details
Published in:In vitro cellular & developmental biology. Animal 2010-07, Vol.46 (7), p.600-605
Main Authors: Shao, Yi, Quyang, Luowa, Zhou, Yueping, Tang, Jing, Tan, Yuehui, Liu, Qiuping, Lin, Zhirong, Yin, Tingting, Qiu, Fangfang, Liu, Zuguo
Format: Article
Language:English
Subjects:
Animal Genetics and Genomics
Animals
Biocompatibility
Biocompatible Materials - metabolism
Biodegradation
Biomaterials
Biomedical and Life Sciences
BIOTECHNOLOGY
Cell Biology
Cell Culture
Cell Proliferation
Cells, Cultured
Cornea
Cornea - cytology
Cornea - metabolism
Corneal Stroma - cytology
Cytotoxicity
Developmental Biology
Epithelial cells
Epithelium, Corneal - cytology
Extracellular Matrix - metabolism
Heterologous transplantation
Humans
Life Sciences
Materials Testing
Porosity
Scaffolds
Sincalide - metabolism
Stem Cells
Sus scrofa
Tissue engineering
Tissue Engineering - methods
Tissue grafting
Water - metabolism
Water uptake
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Summary:This study was to investigate the stability, physico-mechanical property and biocompatibility of porcine corneal acellularized matrix (PCAM) that was prepared using human sera treatment to decellularize corneas. The stability (the rate of biodegradation) and physico-mechanical property (water uptake, density, and porosity) of PCAM were not compromised, compared with porcine fresh cornea matrix (PFCM, p > 0.05). The contact and extract cytotoxicity tests with human corneal epithelial cells and human keratocytes showed that PCAM has a good biocompatibility ex vivo and no cytotoxic effect. These results present the ability to create safety scaffolds that function as cornea grafts and provide a novel experimental approach for the study of cornea tissue engineering using acellular porcine cornea.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-010-9328-9