Structural changes of lignocelluloses by a nonionic surfactant, Tween 20, and their effects on cellulase adsorption and saccharification

► Treatment of lignocelluloses with Tween 20 contributed to the formation of 10- to 50-nm pores. ► Tween 20 treatment increased cellulase adsorption rate and amount onto lignocelluloses substantially. ► Structural effect of Tween 20 depended on lignin contents and crystallinity. In this work, we fou...

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Bibliographic Details
Published in:Bioresource technology 2011-10, Vol.102 (20), p.9605-9612
Main Authors: Seo, Dong-June, Fujita, Hirotaka, Sakoda, Akiyoshi
Format: Article
Language:English
Subjects:
Adsorption
Biological and medical sciences
Carbohydrates - chemistry
cell walls
Cellulase
Cellulase - metabolism
Cellulose
Collapse
Diffusion
endo-1,4-beta-glucanase
Fundamental and applied biological sciences. Psychology
Ions
Lignin - chemistry
Lignocellulose
Lignocelluloses
Microscopy, Electron, Scanning
nonionic surfactants
Polysorbates - pharmacology
Saccharification
Surface-Active Agents - chemistry
Tween 20
Walls
X-Ray Diffraction
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Summary:► Treatment of lignocelluloses with Tween 20 contributed to the formation of 10- to 50-nm pores. ► Tween 20 treatment increased cellulase adsorption rate and amount onto lignocelluloses substantially. ► Structural effect of Tween 20 depended on lignin contents and crystallinity. In this work, we found that Tween 20 treatment (0–8mM) contributed to the cell wall collapse of most samples except for those with high lignin contents and high crystallinity. Cell wall collapse contributed to the formation of 10- to 50-nm pores and not only increased the monolayer saturation amount of adsorbed cellulase about 3–3.6 times but also increased the cellulase adsorption rate (De/r2) about 160–880 times. Moreover, cellulose conversion at 72h was also increased 8.7–21.5% by Tween 20 treatment. On the other hand, the adsorption of Tween 20 on Avicel (microcrystalline cellulose) hindered the cellulase reaction (adsorption and saccharification). The effect of Tween 20 treatment on the crystalline part was insignificant for both lignocelluloses and Avicel. It was found that some degree of pretreatment (e.g. lignin removal) that enhances Tween 20 diffusion into samples is necessary to obtain the structural effects of Tween 20.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2011.07.034