Multiplex PCR for rapid diagnosis of tuberculous meningitis

Rapid and specific diagnosis of tubercular meningitis is of paramount importance to decrease morbidity and mortality. The aim of the study was to evaluate multiplex PCR using protein b, MPB 64, and IS6110 primers directed against M. tuberculosis complex for the diagnosis of tuberculous meningitis (T...

Full description

Saved in:
Bibliographic Details
Published in:Journal of neurology 2011-10, Vol.258 (10), p.1781-1787
Main Authors: Kusum, Sharma, Aman, Sharma, Pallab, Ray, Kumar, Sharma Shiv, Manish, Modi, Sudesh, Prabhakar, Subhash, Varma, Meera, Sharma
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Bacterial diseases
Bacterial diseases of the nervous system. Bacterial myositis
Biological and medical sciences
Child
Child, Preschool
DNA, Bacterial - cerebrospinal fluid
Fever
Genes
Glucose
Guillain-Barre syndrome
Head injuries
Human bacterial diseases
Humans
Infant
Infectious diseases
Medical education
Medical sciences
Medicine
Medicine & Public Health
Meningitis
Microscopy
Middle Aged
Multiplex Polymerase Chain Reaction - methods
Mycobacterium
Mycobacterium tuberculosis - isolation & purification
Neurology
Neuroradiology
Neurosciences
Original Communication
Proteins
Sensitivity and Specificity
Tuberculosis
Tuberculosis, Meningeal - cerebrospinal fluid
Tuberculosis, Meningeal - diagnosis
Viral infections
Young Adult
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Rapid and specific diagnosis of tubercular meningitis is of paramount importance to decrease morbidity and mortality. The aim of the study was to evaluate multiplex PCR using protein b, MPB 64, and IS6110 primers directed against M. tuberculosis complex for the diagnosis of tuberculous meningitis (TBM). Multiplex PCR was performed on 18 TBM confirmed cases (culture was positive), 92 clinically suspected TBM cases and 100 non-TBM (control group) patients. Multiplex PCR had a sensitivity of 94.4% for confirmed cases and specificity of 100% for confirmed TBM cases. In 92 clinically diagnosed but unconfirmed TBM cases, multiplex PCR was positive in 84.78% cases. The overall sensitivity of microscopy, culture and multiplex cases were 1.81, 16.73, and 86.63% and specificity was 100, 100, and 100% respectively. Multiplex PCR using protein b, MPB 64, and IS6110 primers has a high sensitivity and specificity in diagnosis of tubercular meningitis.
ISSN:0340-5354
1432-1459
DOI:10.1007/s00415-011-6010-4