Efficient conversion from polysialogangliosides to monosialotetrahexosylganglioside using Oerskovia xanthineolytica YZ-2

• Published in: Bioprocess and biosystems engineering 2011-05, Vol.34 (4), p.493-498
• Main Authors: Zhang, Jianguo, Cao, Dan, Shen, Danhong, Wang, Xuedong, Wei, Dongzhi
• Format: Article
• Language: English
• Subjects: Actinomycetales - metabolism; Animals; Biological and medical sciences; Bioreactors; Biotechnology; Biotechnology - methods; Carbohydrate Sequence; Chemistry; Chemistry and Materials Science; Culture Media - chemistry; Environmental Engineering/Biotechnology; Fermentation; Food Science; Fundamental and applied biological sciences. Psychology; G(M1) Ganglioside - chemistry; Gangliosides - chemistry; Humans; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Models, Chemical; Molecular Sequence Data; N-Acetylneuraminic Acid - chemistry; Neuraminidase - chemistry; Oerskovia xanthineolytica; Original Paper; RNA, Ribosomal, 16S - genetics; Soils; Swine; Temperature
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-010-0493-8

A new sialidase-producing strain isolated from soil was identified as Oerskovia xanthineolytica YZ-2 . Sialidase was produced when Oerskovia xanthineolytica YZ-2 was exposed to polysialogangliosides. The sialidase of Oerskovia xanthineolytica YZ-2 hydrolyzed sialic acid linkages in polysialogangliosides, and released monosialotetrahexosylganglioside (GM1). The sialidase had the capability of product specificity because it did not attack the sialic acid linkage in GM1. Therefore, Oerskovia xanthineolytica YZ-2 was used for GM1 production from polysialogangliosides. In flasks cultivation phase, it was proved that Oerskovia xanthineolytica YZ-2 could convert polysialogangliosides to GM1 efficiently. Scaling-up the bioprocess with 8% crude ganglioside, polysialogangliosides was converted to GM1 by Oerskovia xanthineolytica YZ-2 in 30 L bioreactor after 18 h. The relative content of GM1 increased from 16.3% in crude ganglioside to 83.7% after Oerskovia xanthineolytica YZ-2 conversion. Therefore, a simple, large-scale conversion process for GM1 production from polysialogangliosides was achieved using O erskovia xanthineolytica YZ-2 as a biocatalyst.