Pre-spawning water temperature affects sperm respiration and reactivation parameters in male carps

Concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. Group 1 was maintained for 7 days at 15°C (cold water (CW) group), whereas the second group was subjected to a temper...

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Bibliographic Details
Published in:Fish physiology and biochemistry 2009-11, Vol.35 (4), p.661-668
Main Authors: Boryshpolets, Sergey, Dzyuba, Boris, Drokin, Sergey
Format: Article
Language:English
Subjects:
Animal Anatomy
Animal Biochemistry
Animal Physiology
Animals
Biomedical and Life Sciences
Carps - physiology
Cell Respiration - physiology
Freshwater
Freshwater & Marine Ecology
Histology
Life Sciences
Male
Morphology
Osmotic pressure
Oxygen Consumption - physiology
Respiration
Spawning
Sperm Motility - physiology
Spermatozoa - physiology
Statistics, Nonparametric
Temperature
Time Factors
Water temperature
Zoology
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Summary:Concentration, ability to motility, motility during the second activation (reactivation), and endogenous respiration were studied in sperm from two experimental groups of carp males. Group 1 was maintained for 7 days at 15°C (cold water (CW) group), whereas the second group was subjected to a temperature of 20°C (warm water (WW) group) before sperm sampling. Reactivation were achieved after incubation of firstly activated sperm in media with osmotic pressure adjusted up to 300 mOsm*kg⁻¹ by increasing K⁺ concentration. Statistically significant reduction of spermatozoa concentration in CW samples versus WW (from 46.0 ± 12.5 (15°C) to 59.3 ± 7 10⁹ (20°C) spermatozoa /ml) have been observed. The sperm of the CW group required a significantly longer incubation time (37 min) under isotonic conditions to achieve a maximum percentage of potent motility at repeated activation than the WW group (23 min). After activation of sperm motility, an increase of respiration rate up to maximum level has been found, this level remained the same under condition of recovering the potential to repeated activation. During the sperm movement respiration rate, in CW group (6.1 nmolO₂/min/10⁹spermatozoa) and WW (3.9 nmolO₂/min/10⁹spermatozoa), was significant higher compared to nonactivated sperm (2.4 nmolO₂/min/10⁹spermatozoa for CW and 1.1 nmolO₂ /min/10⁹spermatozoa for WW). And keeping males for 7 days at 15°C increase the respiration rate of sperm.
ISSN:0920-1742
1573-5168
DOI:10.1007/s10695-008-9292-4