Isolation, identification and characterization of algicidal bacteria against Stephanodiscus hantzschii and Peridinium bipes for the control of freshwater winter algal blooms

Five strains (HYY0510-SK04, HYY0511-SK09, HYK0512-SK12, HYK0512-PK04 and HYY0512-PK05) of algicidal bacteria against the harmful bloom forming diatom Stephanodiscus hantzschii and dinoflagellate Peridinium bipes, were isolated. Among these strains, HYY0510-SK04, HYY0511-SK09 and HYK0512-SK12 have an...

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Published in:Journal of applied phycology 2008-08, Vol.20 (4), p.375-386
Main Authors: Kang, Yoon-Kyung, Cho, Soo-Yeon, Kang, Yoon-Ho, Katano, Toshiya, Jin, Eon-Seon, Kong, Dong-Soo, Han, Myung-Soo
Format: Article
Language:English
Subjects:
Acidovorax delafieldii
Algal bloom
Algicidal bacteria
Bacillariophyceae
Bacteria
Biomedical and Life Sciences
Diatom
Dinoflagellate
Ecology
Freshwater & Marine Ecology
Hydrogenophaga
Life Sciences
Peridinium bipes
Plant Physiology
Plant Sciences
Pseudomonas plecoglossicida
Stephanodiscus hantzschii
Variovorax paradoxus
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Summary:Five strains (HYY0510-SK04, HYY0511-SK09, HYK0512-SK12, HYK0512-PK04 and HYY0512-PK05) of algicidal bacteria against the harmful bloom forming diatom Stephanodiscus hantzschii and dinoflagellate Peridinium bipes, were isolated. Among these strains, HYY0510-SK04, HYY0511-SK09 and HYK0512-SK12 have an effective algicidal activity for S. hantzschii, while HYK0512-PK04 and HYY0512-PK05 have an algicidal effect against P. bipes. Sequence analysis of 16S rDNA showed that HYY0510-SK04 and HYY0511-SK09 were closely related to Acidovorax delafieldii ATCC 17505T. HYK0512-SK12, HYK0512-PK04 and HYY0512-PK05 showed high homology with Variovorax paradoxus IAM 12373T (98.9%), Hydrogenophaga palleronii ATCC 49743T (98.8%) and Pseudomonas plecoglossicida ATCC 700383T (98.3%), respectively. HYY0510-SK04, HYY0511-SK09 and HYK0512-SK12 degraded S. hantzschii cells within two weeks when those bacteria were inoculated at densities of >=10⁷cells mL⁻¹ to the lag or logarithmic growth phase of the algal culture. HYK0512-PK04 and HYY0512-PK05 degraded more than 90% of P. bipes cells within 14 and 8 days, respectively, when these bacteria were inoculated at densities of >=10⁷cells mL⁻¹. Among the five bacterial strains, HYK0512-SK12 and HYY0512-PK05 showed the most effective growth inhibition of all the algae and cyanobacteria tested. Biochemical assays revealed that the main algicidal substance from all isolates were likely to be extracellular substances. These results indicate that the bacterial strains isolated for this study are potential agents for the control of harmful algal blooms in eutrophic reservoirs.
ISSN:0921-8971
1573-5176
DOI:10.1007/s10811-007-9267-3