Drugs of Abuse Detection in Meconium: A Comparison Between ELISA and Biochip Microarray

The results of meconium specimens and fortified samples screened for drugs of abuse by both enzyme-linked immunosorbent assay (ELISA, Immunalysis) and biochip microarray (Randox) methods were compared. The ELISA method was semi-automated using a TECAN Genesis. The Randox assay used the Randox Eviden...

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Bibliographic Details
Published in:Journal of analytical toxicology 2011, Vol.35 (1), p.40-45
Main Authors: Marin, Stephanie J., Merrell, Miles, McMillin, Gwendolyn A.
Format: Article
Language:English
Subjects:
Amphetamine
biochips
Biological and medical sciences
Cannabinoids
Cannabinoids - analysis
Chromatography, Liquid
Cocaine
Cocaine - analysis
Drug abuse
Drug addictions
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Gas chromatography
Gas Chromatography-Mass Spectrometry
Humans
Infant, Newborn
Mass spectroscopy
Meconium
Meconium - chemistry
Medical sciences
Methamphetamine - analysis
Protein Array Analysis - methods
Quantitation
Street Drugs - analysis
Substance Abuse Detection - methods
Tandem Mass Spectrometry
Toxicology
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Summary:The results of meconium specimens and fortified samples screened for drugs of abuse by both enzyme-linked immunosorbent assay (ELISA, Immunalysis) and biochip microarray (Randox) methods were compared. The ELISA method was semi-automated using a TECAN Genesis. The Randox assay used the Randox Evidence Investigator system. Previously validated gas chromatography-mass spectrometry (GC-MS), GC-GC-MS, or liquid chromatography-MS-MS methods were used for confirmation and quantitation. Results from the two techniques compared well. Agreement of the Randox assay was greater than 90% when compared to the ELISA assay for all drug classes except cannabinoids (88%). Specificity of the biochip assay was slightly better for amphetamines and cocaine.
ISSN:0146-4760
1945-2403
DOI:10.1093/anatox/35.1.40