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Disulfide bond formation and its impact on the biological activity and stability of recombinant therapeutic proteins produced by Escherichia coli expression system

Therapeutic proteins require correct disulfide bond formation for biological activity and stability. This makes their manufacturing and storage inherently challenging since disulfide bonds can be aberrantly formed and/or undergo significant structural changes. In this paper the mechanisms of disulfi...

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Published in:	Biotechnology advances 2011-11, Vol.29 (6), p.923-929
Main Authors:	Zhang, Lin, Chou, C. Perry, Moo-Young, Murray
Format:	Article
Language:	English
Subjects:	bioactive properties Biological activity Biological and medical sciences biopharmaceuticals Biotechnology Biotechnology - methods Disulfide bond formation/scrambling disulfide bonds Disulfides - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology manufacturing Protein Stability recombinant proteins Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant therapeutic protein Stability storage quality
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creator	Zhang, Lin Chou, C. Perry Moo-Young, Murray
description	Therapeutic proteins require correct disulfide bond formation for biological activity and stability. This makes their manufacturing and storage inherently challenging since disulfide bonds can be aberrantly formed and/or undergo significant structural changes. In this paper the mechanisms of disulfide bond formation and scrambling are reviewed, with a focus on their impact on the biological activity and storage stability of recombinant proteins. After assessing the research progress in detecting disulfide bond scrambling, strategies for preventing this phenomenon are proposed.
doi_str_mv	10.1016/j.biotechadv.2011.07.013
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subjects	bioactive properties Biological activity Biological and medical sciences biopharmaceuticals Biotechnology Biotechnology - methods Disulfide bond formation/scrambling disulfide bonds Disulfides - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fundamental and applied biological sciences. Psychology manufacturing Protein Stability recombinant proteins Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant therapeutic protein Stability storage quality
title	Disulfide bond formation and its impact on the biological activity and stability of recombinant therapeutic proteins produced by Escherichia coli expression system
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