Quantitative trait loci analysis for resistance to Cephalosporium stripe, a vascular wilt disease of wheat

• Published in: Theoretical and applied genetics 2011-05, Vol.122 (7), p.1339-1349
• Main Authors: Quincke, Martin C, Peterson, C. James, Zemetra, Robert S, Hansen, Jennifer L, Chen, Jianli, Riera-Lizarazu, Oscar, Mundt, Christopher C
• Format: Article
• Language: English
• Subjects: Acremonium - growth & development; Acremonium - pathogenicity; additive effect; Agricultural production; Agriculture; alleles; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Cephalosporium; Cephalosporium gramineum; Chromosome Mapping; chromosomes; Chromosomes, Plant; Classical genetics, quantitative genetics, hybrids; conservation tillage; Crop diseases; Crosses, Genetic; Cultivars; DNA, Plant - isolation & purification; Fundamental and applied biological sciences. Psychology; Genes, Plant; Genetic aspects; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; grain yield; Hymenula cerealis; Immunity, Innate; inbred lines; Life Sciences; linkage groups; microsatellite repeats; Original Paper; Pathogens; Physiological aspects; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Polymorphism, Genetic; Pteridophyta, spermatophyta; Quantitative Trait Loci; regression analysis; Science; screening; Soil sciences; Triticum - genetics; Triticum - immunology; Triticum - microbiology; Triticum aestivum; vascular wilt; Vegetals; Wheat; wilting
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-011-1535-6

Cephalosporium stripe, caused by Cephalosporium gramineum, can cause severe loss of wheat (Triticum aestivum L.) yield and grain quality and can be an important factor limiting adoption of conservation tillage practices. Selecting for resistance to Cephalosporium stripe is problematic; however, as optimum conditions for disease do not occur annually under natural conditions, inoculum levels can be spatially heterogeneous, and little is known about the inheritance of resistance. A population of 268 recombinant inbred lines (RILs) derived from a cross between two wheat cultivars was characterized using field screening and molecular markers to investigate the inheritance of resistance to Cephalosporium stripe. Whiteheads (sterile heads caused by pathogen infection) were measured on each RIL in three field environments under artificially inoculated conditions. A linkage map for this population was created based on 204 SSR and DArT markers. A total of 36 linkage groups were resolved, representing portions of all chromosomes except for chromosome 1D, which lacked a sufficient number of polymorphic markers. Quantitative trait locus (QTL) analysis identified seven regions associated with resistance to Cephalosporium stripe, with approximately equal additive effects. Four QTL derived from the more susceptible parent (Brundage) and three came from the more resistant parent (Coda), but the cumulative, additive effect of QTL from Coda was greater than that of Brundage. Additivity of QTL effects was confirmed through regression analysis and demonstrates the advantage of accumulating multiple QTL alleles to achieve high levels of resistance.