Simple hormonal regulation of somatic embryogenesis and/or shoot organogenesis in caryopsis cultures of Pogonatherum paniceum (Poaceae)

• Published in: Plant cell, tissue and organ culture tissue and organ culture, 2008-10, Vol.95 (1), p.57-67
• Main Authors: Wang, Wenguo, Zhao, Xiaoguang, Zhuang, Guoqing, Wang, Shenghua, Chen, Fang
• Format: Article
• Language: English
• Subjects: 2,4-D; benzyladenine; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; callus formation; conservation plants; culture media; Fundamental and applied biological sciences. Psychology; grass seed; Life Sciences; methodology; micropropagation; naphthaleneacetic acid; new methods; nursery crops; organogenesis; Original Paper; ornamental grasses; perennials; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Poaceae; Pogonatherum paniceum; regeneration pathways; somatic embryogenesis; sucrose
• ISSN: 0167-6857; 1573-5044
• DOI: 10.1007/s11240-008-9414-9

Pogonatherum paniceum (Poaceae) is a perennial plant with good potential for eco-recovery and ornamental function. This study presents in vitro culture systems of simple hormonal regulation of somatic embryogenesis and shoot organogenesis from mature caryopses. Mature caryopses of P. paniceum were grown on Murashige and Skoog medium with 3% sucrose (w/v) and various concentrations or combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), α-naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP). Morphological development was analyzed by light microscope after histological sectioning. Four types of callus were induced by different concentrations of 2,4-D. Type I callus was regenerated via somatic embryogenesis; type II callus failed to produce any regeneration; type III callus had both somatic embryogenesis and shoot organogenesis capacities; and type IV callus only displayed shoot organogenesis capacity. Regarding hormone combinations used in this study, NAA only induced type IV callus and BAP only induced direct multiple shoot formation. The combinations of 2,4-D and NAA induced type III callus. Several of the regeneration pathways were simply controlled by one or two kinds of plant hormones. The established systems will be helpful for further research on the developmental mechanism of switch between somatic embryogenesis and shoot organogenesis.