The influence of low pH on in vitro growth and biochemical parameters of Plantago almogravensis and P. algarbiensis

The effects of low medium pH (4.50, 5.00 and 5.75) on in vitro growth and on several biochemical parameters (lipid peroxidation, proline and carbohydrate content, antioxidant enzymes activities and total soluble protein) of Plantago almogravensis and P. algarbiensis micropropagated shoots were inves...

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Bibliographic Details
Published in:Plant cell, tissue and organ culture tissue and organ culture, 2011-10, Vol.107 (1), p.113-121
Main Authors: Martins, Neusa, Gonçalves, Sandra, Palma, Tânia, Romano, Anabela
Format: Article
Language:English
Subjects:
Acidic soils
Antioxidants
Biological and medical sciences
Biomedical and Life Sciences
Biotechnology
Carbohydrates
Cell culture
Damage accumulation
Enzymes
Fundamental and applied biological sciences. Psychology
Life Sciences
Lipid peroxidation
Lipids
Organ culture
Original Paper
Parameters
Peroxidation
pH effects
Plant Genetics and Genomics
Plant Pathology
Plant Physiology
Plant Sciences
Plantago
Plasma membranes
Proline
Rooting
Roots
Shoots
Soils (acid)
Species
Tissue culture
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Summary:The effects of low medium pH (4.50, 5.00 and 5.75) on in vitro growth and on several biochemical parameters (lipid peroxidation, proline and carbohydrate content, antioxidant enzymes activities and total soluble protein) of Plantago almogravensis and P. algarbiensis micropropagated shoots were investigated. Overall, it was observed that medium pH did not affect in vitro proliferation and rooting. Interestingly, cultures of both species modify the initial pH value to the same final value. Results have shown that the lowest pH tested induced an increase in the level of lipid peroxidation in roots of both species and in shoots of P. algarbiensis , indicating plasma membrane damage. An accumulation of carbohydrates was observed in roots of P. almogravensis cultured in pH 4.50 and 5.00. It was observed a slight response of the enzymatic system to medium pH, particularly in P. almogravensis . Based on the results obtained we can conclude that Plantago species are apt to grow in vitro in medium with pH values much lower than the usually used in tissue culture, which is in agreement with the fact that both species colonize acid soils.
ISSN:0167-6857
1573-5044
DOI:10.1007/s11240-011-9963-1