Field guide to next-generation DNA sequencers

The diversity of available 2nd and 3rd generation DNA sequencing platforms is increasing rapidly. Costs for these systems range from $10/Mb for 454 and some Ion Torrent chips). In terms of cost per nonmultiplexed sample and instrument run time, the Pacific Biosciences and Ion Torrent platforms excel...

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Bibliographic Details
Published in:Molecular ecology resources 2011-09, Vol.11 (5), p.759-769
Main Author: GLENN, TRAVIS C.
Format: Article
Language:English
Subjects:
2nd and 3rd generation sequencing
Computational Biology - methods
Deoxyribonucleic acid
DNA
Helicos
Illumina
Ion Torrent
Life Technologies
massively parallel sequencing
Pacific Biosystems
Roche
Sequence Analysis, DNA - economics
Sequence Analysis, DNA - instrumentation
Sequence Analysis, DNA - methods
SOLiD
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Summary:The diversity of available 2nd and 3rd generation DNA sequencing platforms is increasing rapidly. Costs for these systems range from $10/Mb for 454 and some Ion Torrent chips). In terms of cost per nonmultiplexed sample and instrument run time, the Pacific Biosciences and Ion Torrent platforms excel, with the 454 GS Junior and Illumina MiSeq also notable in this regard. All platforms allow multiplexing of samples, but details of library preparation, experimental design and data analysis can constrain the options. The wide range of characteristics among available platforms provides opportunities both to conduct groundbreaking studies and to waste money on scales that were previously infeasible. Thus, careful thought about the desired characteristics of these systems is warranted before purchasing or using any of them. Updated information from this guide will be maintained at: http://dna.uga.edu/ and http://tomato.biol.trinity.edu/blog/.
ISSN:1755-098X
1755-0998
DOI:10.1111/j.1755-0998.2011.03024.x