Expression of matrix metalloproteinases 1, 3, and 9 in differing extents of tendon retraction in the torn rotator cuff

• Published in: Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA sports traumatology, arthroscopy : official journal of the ESSKA, 2011-10, Vol.19 (10), p.1760-1765
• Main Authors: Lakemeier, Stefan, Braun, Juliane, Efe, Turgay, Foelsch, Christian, Archontidou-Aprin, Eleni, Fuchs-Winkelmann, Susanne, Paletta, Juergen R. J., Schofer, Markus D.
• Format: Article
• Language: English
• Subjects: Aged; Case-Control Studies; Enzymes; Extracellular matrix; Female; Health; Humans; Knees; Male; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase 3 - metabolism; Matrix Metalloproteinase 9 - metabolism; Medicine; Medicine & Public Health; Middle Aged; Orthopedics; Patients; Polyclonal antibodies; Rotator cuff; Rotator Cuff - enzymology; Rotator Cuff - pathology; Rotator Cuff - surgery; Rotator Cuff Injuries; Shoulder; Sports; Surgery; Tendon Injuries - enzymology; Tendon Injuries - surgery; Tendons
• ISSN: 0942-2056; 1433-7347
• DOI: 10.1007/s00167-010-1367-y

Purpose Differing extents of tendon retraction are found in full-thickness rotator cuff tears. The pathophysiologic context of tendon degeneration and the extent of tendon retraction are unclear. Tendon integrity depends on the extracellular matrix, which is regulated by matrix metalloproteinases (MMP). It is unknown which enzymes play a role in tendon degeneration. The hypotheses are that (1) the expression of MMPs 1, 3, and 9 is altered in the torn rotator cuff when compared with healthy tendon samples; and (2) that there is a relationship between MMP expression and the extent of tendon retraction in the torn cuff. Methods Rotator cuff tendon samples of 33 patients with full-thickness rotator cuff tears (Bateman grade III) were harvested during reconstructive surgery. Samples were dehydrated and paraffin-embedded. Immunohistologic determination of MMP 1, 3, and 9 expression was performed by staining sample slices with MMP antibody. The extent of tendon retraction was determined intraoperatively according to Patte’s classification and patients were assigned to 4 groups (control group, and by tendon retraction grade Patte I-III). The control group consisted of six healthy tendon samples. Results Expression of MMPs 1 and 9 was significantly higher in torn cuff samples than in healthy tendons whereas MMP 3 expression was significantly decreased ( P